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  • © 1998

Plant Virology Protocols

From Virus Isolation to Transgenic Resistance

Part of the book series: Methods in Molecular Biology (MIMB, volume 81)

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Table of contents (55 protocols)

  1. Front Matter

    Pages i-xv
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Introduction to Plant Virology

      • Bradley I. Hillman
      Pages 3-12
    3. Introduction to Classical Crossprotection

      • Ron S. S. Fraser
      Pages 13-24
    4. History of Coat Protein-Mediated Protection

      • Eric D. Miller, Cynthia Hemenway
      Pages 25-38
  3. Virus Isolation, RNA Extraction, and Location of Coat Protein Genes

    1. Front Matter

      Pages 39-39
    2. Geminivirus Isolation and DNA Extraction

      • Kenneth E. Palmer, Wendelin H. Schnippenkoetter, Edward P. Rybicki
      Pages 41-52
    3. Caulimovirus Isolation and DNA Extraction

      • Simon N. Covey, Rob J. Noad, Nadia S. Al-Kaff, David S. Turner
      Pages 53-63
    4. Reovirus Isolation and RNA Extraction

      • Ichiro Uyeda, Bong-Choon Lee, Yuko Ando, Haruhisa Suga, Yun-Kun He, Masamichi Isogai
      Pages 65-75
    5. Hordeivirus Isolation and RNA Extraction

      • Diane M. Lawrence, Andrew O. Jackson
      Pages 99-106
    6. Furovirus Isolation and RNA Extraction

      • Salah E. Bouzoubaa
      Pages 107-114
    7. Tobravirus Isolation and RNA Extraction

      • Sally C. Taylor
      Pages 115-121
    8. Tobamovirus Isolation and RNA Extraction

      • Sean N. Chapman
      Pages 123-129
    9. Potexvirus Isolation and RNA Extraction

      • Mounir G. AbouHaidar, Huimin Xu, Kathleen L. Hefferon
      Pages 131-143
    10. Carlavirus Isolation and RNA Extraction

      • Gary D. Foster
      Pages 145-150
    11. Potyvirus Isolation and RNA Extraction

      • Philip H. Berger, Patrick J. Shiel
      Pages 151-160
    12. Trichovirus Isolation and RNA Extraction

      • Sylvie German-Retana, Thierry Candresse, Jean Dunez
      Pages 161-170
    13. Ilarvirus Isolation and RNA Extraction

      • Deyin Guo, Edgar Maiss, Günter Adam
      Pages 171-181
    14. Bromovirus Isolation and RNA Extraction

      • Jozef Julian Bujarski
      Pages 183-188

About this book

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

Editors and Affiliations

  • Plant Virology Group School of Biological Sciences, University of Bristol, UK

    Gary D. Foster

  • Department of Botany, University of Leicester, UK

    Gary D. Foster, Sally C. Taylor

Bibliographic Information

  • Book Title: Plant Virology Protocols

  • Book Subtitle: From Virus Isolation to Transgenic Resistance

  • Editors: Gary D. Foster, Sally C. Taylor

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033856

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • Hardcover ISBN: 978-0-89603-385-6Published: 24 February 1998

  • Softcover ISBN: 978-1-4899-4253-1Published: 14 August 2013

  • eBook ISBN: 978-1-59259-566-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 571

  • Number of Illustrations: 111 b/w illustrations

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access