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DNA Sequencing Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Colin A. Graham

    1. Northern Ireland Regional Genetics Centre, Belfast City Hospital Trust, Northern Ireland, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Alison J. M. Hill

    1. Department of Anatomy and Physiology, University of Dundee, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 167)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to DNA Sequencing

    • Colin A. Graham, Alison J. M. Hill
    Pages 1-12

  3. The Universal Primers and the Shotgun DNA Sequencing Method

    • Joachim Messing
    Pages 13-31

  4. M13 Sequencing

    • Qingzhong Yu
    Pages 33-38

  5. Primer Design and Primer-Directed Sequencing

    • Ulrike Gerischer, Peter Dürre
    Pages 39-51

  6. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction

    • Ulrike Gerischer
    Pages 53-61

  7. Solid Phase Fluorescent Sequencing of the CFTR Gene

    • Harry Cuppens, Jean-Jacques Cassiman
    Pages 63-88

  8. Shotgun DNA Sequencing

    • Alan T. Bankier
    Pages 89-100

  9. Cycle Sequencing

    • Robert W. Blakesley
    Pages 101-112

  10. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers

    • Johannes Zschocke, Georg F. Hoffmann
    Pages 113-117

  11. Automated Fluorescent DNA Sequencing on the ABI PRISM 377

    • Jonathan R. E. MacBeath, Suzanna S. Harvey, Nicola J. Oldroyd
    Pages 119-152

  12. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer

    • David Watts, Jonathan R. E. MacBeath
    Pages 153-170

  13. Fluorescent Sequencing Protocols for the ALF

    • Shirley McQuaid
    Pages 171-192

  14. Fluorescent Sequencing for Heterozygote Mutation Detection

    • Colin A. Graham, Alison J. M. Hill
    Pages 193-213

  15. Sequence Databases and the Internet

    • Anne E. Hughes
    Pages 215-223

  16. DNA Sequencing by Capillary Array Electrophoresis

    • Norman J. Dovichi, JianZhong Zhang
    Pages 225-239

  17. Back Matter

    Pages 241-244
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About this book

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Reviews

From Reviews of the First Edition

"...continues the standard of excellent laboratory manuals that has been set by earlier volumes...Many of the protocols are significantly easier to follow than comparable protocols in other lab handbooks...provides one of the best collections of manuals for DNA sequencing applications..." -FEBS Letters
"...a valuable addition to the library of any laboratory that is actively engaged in DNA sequencing..." -Quarterly Review of Biology
"...any laboratory undertaking a large DNA sequencing project...would benefit from having this manual..." -Trends in Biotechnology

Editors and Affiliations

  • Northern Ireland Regional Genetics Centre, Belfast City Hospital Trust, Northern Ireland, UK

    Colin A. Graham

  • Department of Anatomy and Physiology, University of Dundee, Scotland, UK

    Alison J. M. Hill

Bibliographic Information

  • Book Title: DNA Sequencing Protocols

  • Editors: Colin A. Graham, Alison J. M. Hill

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591132

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • Softcover ISBN: 978-1-4899-4212-8Published: 01 September 2013

  • eBook ISBN: 978-1-59259-113-8Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 244

  • Number of Illustrations: 33 b/w illustrations

  • Topics: Cell Biology

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