In Vitro Toxicity Testing Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Alternative Method of Assessing Toxicity

   1. Alternative Method of Assessing Toxicity

      • Chris K. Atterwill

      Pages 1-9

3. Target Organ Toxicity

   1. LLC-RK1 Cell Screening Test for Nephrotoxicity

      • David J. White, Chris Seaman

      Pages 11-16

   2. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity

      • Mark R. Cookson, R. McClean, Vietor W. Pentreatk

      Pages 17-23

   3. Human Thyroid Culture

      • Carmel Mothersill

      Pages 25-31

   4. Thyroid Follicular Cells in Monolayer Culture

      • Stephen P. Bidey

      Pages 33-42

   5. Dust Toxicity in Rat Alveolar Macrophage Cultures

      • Yrjö Collan, Veli- Matti Kosma

      Pages 43-49

   6. Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity

      • Margherita Ferro

      Pages 51-57

   7. Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytes

      • Giovanni Brambilla, Antonietta Martelli

      Pages 59-66

   8. Model Cavity Method

      • Paul A. Duffy

      Pages 67-74

   9. Human Esophageal Culture

      • Carmel Mothersill

      Pages 75-79

   10. The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing

       • Glenda E. Gillies, Julia C. Buckingham

       Pages 81-93

   11. The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing

       • Glenda E. Gillies, Julia C. Buckingham

       Pages 95-107

4. General and Topical Toxicity

   1. The FRAME Cytotoxicity Test (Kenacid Blue)

      • Richard H. Clothier

      Pages 109-118

   2. Allium Test

      • Geirid Fiskesjö

      Pages 119-127

   3. Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose

      • Erik Walum, Anna Forsby

      Pages 129-135

   4. MTT Assays

      • Rosa Supino

      Pages 137-149

   5. V79 Cytotoxicity Test for Membrane Damage

      • Vera Bianchi

      Pages 151-160

   6. SIRC Cytotoxicity Test

      • Odile Blein-Sella, Monique Adolphe

      Pages 161-167

   7. Rabbit Articular Chondrocyte Functional Toxicity Test

      • Odile Blein-Sella, Monique Adolphe

      Pages 169-175

In vitro toxicology is one of the most rapidly expanding areas of biological research today. It is generally conceded that this is a result of pressure from the public for safer products and environmental conditions and, in these recessionary times, pressure from company accountants, who often perceive in vitro experimentation as a cheaper option than lawsuits, fines, and expensive remediation. Toxicologists themselves form one group whose vigor and influence on the evolution of in vitro toxicology is often underestimated. Many toxicologists have for some time been unhappy with the quantity (often a result of unnecessary duplication), the reliability, and the ethical circumstances of much animal experimentation. It is hoped that the present book, In Vitro Toxicity Testing Protocols, will play some part in enabling and encouraging the more widespread use of in vitro techniques. Since in vitro toxicity testing methodology is evolving at such a rapid rate, this book can of necessity provide only a "snapshot" of the major techniques in use at the present time. As with the other publications in Humana Press' Methods in Molecular Biology series, the aim has been to supply information on the fundamental requirements for the establishment of new techniques within working laboratories and to describe a variety of basic in vitro methodologies. Certain of the techniques detailed here could more accurately be described as ex vivo rather than truly in vitro. No apology is made for the inclusion of these techniques.

...an excellent presentation of the recent advances made in in-vitro toxicology...authors place these advances in a meaningful perspective and present the material in easy to follow protocol...the authors succeeded in their efforts to fill the need for current knowledge on this subject.****-Doody's Health Sciences Book Review Journal

• University of Hertfordshire, Hatfield, UK

  Sheila O’Hare, Chris K. Atterwill

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