Intracellular Messengers

1. Front Matter

   Pages i-xix

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2. Identification and Analysis of Function of Heterotrimeric Guanine Nucleotide-Binding Proteins Expressed in Neural Tissue

   • Graeme Milligan

   Pages 1-56

3. Methods for the Analysis of Phosphoinositides and Inositol Phosphates

   • Philip P. Godfrey

   Pages 57-78

4. Inositol Triphosphate Receptors and Intracellular Calcium

   • Colin W. Taylor, Jennifer M. Bond, David L. Nunn, Katherine A. Oldershaw

   Pages 79-128

5. Measurement of Intracellular Calcium with Fluorescent Calcium Indicators

   • Martin Poenie

   Pages 129-174

6. Single-Cell Imaging Technology

   • Roger B. Moreton

   Pages 175-230

7. Analysis of Protein Kinase C Function

   • Gillian M. Burgess

   Pages 231-272

8. Synthetic Analogs of Intracellular Messengers

   • Barry V. L. Potter

   Pages 273-312

9. Methods in Cyclic Nucleotide Research

   • Kenneth J. Murray

   Pages 313-368

10. Caged Intracellular Messengers and the Inositol Phosphate Signaling Pathway

    • Ian Parker

    Pages 369-396

11. Protein Phosphorylation

    • David B. Morton

    Pages 397-431

12. Investigation of the Intracellular Regulators and Components of the Exocytotic Pathway

    • Robert D. Burgoyne

    Pages 433-470

13. Intracellular Messengers in Vertebrate Photoreceptors

    • Hugh R. Matthews, Trevor D. Lamb

    Pages 471-516

14. Intracellular Messengers in Invertebrate Photoreceptors Studied in Mutant Flies

    • Baruch Minke, Zvi Selinger

    Pages 517-563

15. Back Matter

    Pages 565-580

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Despite the success of earlier Neuromethads volumes, I was initially reluctant to edit a further volume because my own - search is concerned with nonneural tissues. I changed my mind for two simple reasons. First, though the sheer diversity of ext- cellular signal molecules is staggering, still more impressive is the remarkably small number of transmembrane signaling processes they recruit- their receptors either have integral ion channels or enzyme activities, or else they catalytically activate G proteins. Likewise, when we look to the final intracellular t- gets of these signaling pathways, they are stucturally diverse, but again there are common themes: the response may either be the gating of an ion channel, or else the phosphorylation of a target protein. Such conservation of signaling mechanisms is both impressive and convenient, and provides my justification for asking authors with interests in diverse tissues to contribute their methodological expertise to this volume. Second, I think it would be difficult to overestimate the extent to which our understanding of intracellular signaling has been transformed by new and improved methodology. Thus, simple methods for measuring inositol phosphates have revealed the profound and widespread importance of the ph- phoinositide pathways, the techniques of contemporary - lecular biology have provided unrivaled opportunities to relate structure and function, and the complex spatial and t- poral characteristics of intracellular signaling pathways were barely imaginable before the introduction of fluorescent indi- tors and single cell-imaging technology.

An extensive review of current knowledge of intracellular information processing....comprehensive....[Offers] a valuable extension to 'classical' neurobiology and provides new tools in neurochemistry.-Journal of Chemical Neuroanatomy

• University of Saskatchewan, Saskatoon, Canada

  Alan A. Boulton

• University of Alberta, Edmonton, Canada

  Glen B. Baker

• University of Cambridge, Cambridge, UK

  Colin W. Taylor

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