Peptide Analysis Protocols

1. Front Matter

   Pages i-xii

   PDF

2. Gel-Filtration Chromotography

   • Daniel M. Bollag

   Pages 1-9

3. Ion-Exchange Chromatography

   • Daniel M. Bollag

   Pages 11-22

4. Reversed-Phase HPLC

   • Udo Nirenberg

   Pages 23-35

5. Reversed-Phase High-Performance Liquid Chromatography

   • Michael E. Byrnes

   Pages 37-52

6. Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis

   • Dan L. Crimmins

   Pages 53-64

7. Principles and Practice of Peptide Analysis with Capillary Zone Electrophoresis

   • Thomas E. Wheat

   Pages 65-83

8. Fast Atom Bombardment Mass Spectrometric Characterization of Peptides

   • P. R. Das, B. N. Pramanik

   Pages 85-106

9. Sequence Analysis of Peptide Resins from Boc/Benzyl Solid-Phase Synthesis

   • Jan Pohl

   Pages 107-129

10. NMR Spectroscopy of Peptides and Proteins

    • Mark G. Hinds, Raymond S. Norton

    Pages 131-154

11. Techniques for Conjugation of Synthetic Peptides to Carrier Molecules

    • J. Mark Carter

    Pages 155-191

12. Epitope Prediction Methods

    • J. Mark Carter

    Pages 193-206

13. Epitope mapping of a protein using the Geysen (PEPSCAN) Procedure

    • J. Mark Carter

    Pages 207-223

14. Analysis of Proteinase Specificity by Studies of Peptide Substrates

    • Ben M. Dunn, Paula E. Scarborough, Ruth Davenport, Wieslaw Swietnicki

    Pages 225-243

15. Synthesis of Recombinant Peptides

    • Gino Van Heeke, Jay S. Stout, Fred W. Wagner

    Pages 245-260

16. De Novo Design of Proteins

    • Gabriele Tuchscherer, Verena Steiner, Karl- Heinz Altmann, Manfred Mutter

    Pages 261-285

17. Chemical Synthesis of the Aspartic Proteinase from Human Immunodeficiency Virus (HIV)

    • Paul D. Hoeprich Jr.

    Pages 287-304

18. Multiple and Combinatorial Peptide Synthesis

    • Philip C. Andrews, Daniele M. Leonard, Wayne L. Cody, Tomi K. Sawyer

    Pages 305-328

19. Back Matter

    Pages 329-335

    PDF

As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.

...represent(s) excellent value for money, and are highly recommended. -The European Peptide Society Newsletter

• University of Florida, Gainesville

  Ben M. Dunn

• Bachem Bioscience, King of Prussia

  Michael W. Pennington

Policies and ethics