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Methods in Protein Sequence Analysis

  • Book
  • © 1993

Overview

Editors:
  1. Kazutomo Imahori

    1. Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Fumio Sakiyama

    1. Osaka University, Osaka, Japan

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Table of contents (38 chapters)

  1. Front Matter

    Pages i-xvi
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  2. Micropreparation and Microsequencing

    1. Front Matter

      Pages 1-1
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    2. Capillary Liquid Chromatography: A Tool for Protein Structural Analysis

      • Robert L. Moritz, Richard J. Simpson
      Pages 3-10

    3. Routine Amino Acid Sequencing on 2D-Gel Separated Proteins: A Protein Elution and Concentration Gel System

      • J. Vandekerckhove, M. Rider, H.-H. Rasmussen, S. De Boeck, M. Puype, J. Van Damme et al.
      Pages 11-19

    4. Capillary Electrophoresis in Structural Characterization of Polypeptides

      • Tomas Bergman
      Pages 21-28

    5. Sensitization of Gas-Phase Protein Sequencer using Fluorescein Isothiocyanate (FITC)

      • Koji Muramoto, Kiyoshi Nokihara, Akira Ueda, Hisao Kamiya
      Pages 29-36

    6. Thiobenzoylation Method of Protein Sequencing: Gas Chromatography/Mass Spectrometric Detection of 5-Acetoxy-2-Phenylthiazoles

      • Mark L. Stolowitz, Chin-Sook Kim, Steven R. Marsh, Leroy Hood
      Pages 37-44

    7. Deblocking and Subsequent Microsequence Analysis of N-Terminally Blocked Proteins Immobilized on PVDF Membrane

      • Susumu Tsunasawa, Hisashi Hirano
      Pages 45-53

    8. Development of Novel C-Terminal Sequencing Methods

      • Akira Tsugita, Keiji Takamoto, Hiromoto Iwadate, Masaharu Kamo, Hiroyuki Yano, Norifumi Miyatake et al.
      Pages 55-62

    9. Automated C-Terminal Sequencing of Peptides and Proteins

      • Jerome M. Bailey, Miro Rusnak, John E. Shively
      Pages 63-69

    10. A New Chemical Approach to C-Terminal Microsequence Analysis via the Thiohydantoin

      • Adam S. Inglis, Claudio De Luca
      Pages 71-78

    11. Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots

      • Gerhard Frank, Udo Redweik, Monika Rümbeli, Gabriele Schoedon, Nenad Blau
      Pages 79-85

    12. Identification of the Disulfide Bonds of the Human Complement Component C3

      • Johann Schaller, Sabine Söhndel, Egon E. Rickli
      Pages 87-93

    13. A Novel Protease from Jack-Bean Seeds: Asparaginyl Endopeptidase

      • Shin-ichi Ishii, Yukichi Abe, Masanori Mitta, Hideyuki Matsushita, Ikunoshin Kato
      Pages 95-100

    14. Lysine-Specific Serine Protease from Achromobacter Lyticus: Its Substrate Specificity and Comparison with Trypsin

      • Shigemi Norioka, Fumio Sakiyama
      Pages 101-106

    15. Structure and Function of Snake Venom Metalloproteinase Family

      • Sadaaki Iwanaga, Hiroyuki Takeya
      Pages 107-115

  3. Mass Spectrometry

    1. Front Matter

      Pages 117-117
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    2. Recent Advances in Protein Sequencing by Mass Spectrometry Introduction and Overview

      • Klaus Biemann
      Pages 119-126

    3. Sequence Analysis of Peptides Presented to the Immune System by Class I and Class II MHC Molecules

      • Donald F. Hunt, Jeffrey Shabanowitz, Hanspeter Michel, Andrea L. Cox, Tracey Dickinson, Theresa Davis et al.
      Pages 127-133

    4. High Precision Mass Spectrometry with the Ion Trap Mass Spectrometer

      • R. Graham Cooks, Kathleen A. Cox, Jon D. Williams
      Pages 135-141
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Keywords

About this book

The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.

Editors and Affiliations

  • Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan

    Kazutomo Imahori

  • Osaka University, Osaka, Japan

    Fumio Sakiyama

Bibliographic Information

  • Book Title: Methods in Protein Sequence Analysis

  • Editors: Kazutomo Imahori, Fumio Sakiyama

  • DOI: https://doi.org/10.1007/978-1-4899-1603-7

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1993

  • Hardcover ISBN: 978-0-306-44488-3Published: 30 June 1993

  • Softcover ISBN: 978-1-4899-1605-1Published: 29 May 2013

  • eBook ISBN: 978-1-4899-1603-7Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XVI, 310

  • Topics: Biochemistry, general, Biotechnology

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