Cell Membrane Transport

1. Front Matter

   Pages i-xvii

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2. General Methodologies

   1. Front Matter

      Pages 1-1

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   2. Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems

      • Rosa Deves

      Pages 3-19

   3. Carrier Kinetics Show How the Sodium Pump Uses ATP to Render Pumping of Both Sodium and Potassium Effective, and Suggest a Model for the Action of the F0F1 ATP-Ases

      • Wilfred D. Stein

      Pages 21-38

   4. Voltage Dependent Conductances: Gating Currents and Single Channel Recordings

      • Francisco Bezanilla

      Pages 39-56

   5. Methods of Determining the Routes of Intestinal Water Transport

      • R. J. Naftalin

      Pages 57-83

3. Isotopic Methods for Flux Determination in Various Membrane Preparations

   1. Front Matter

      Pages 85-85

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   2. Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired-Tracer Dilution: A Review of Recent Studies

      • David L. Yudilevich, Luis A. Sobrevía, L. Felipe Barros

      Pages 87-106

   3. Some Inexact Methods for the Analysis of Transport Kinetics Using Dual Tracers

      • I. Christopher H. Smith

      Pages 107-112

   4. Transport Studies in Isolated Enterocytes

      • A. Ilundáin

      Pages 113-130

   5. Cell Culture in the Study of Epithelial Development and Function

      • F. V. Sepúlveda

      Pages 131-141

   6. New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique

      • S. Perán, M. Muñoz, M. T. Saiz

      Pages 143-161

   7. Methodological Guide for Studying Epithelial Transport with Isolated Membrane Vesicles

      • J. Biber, H. Murer

      Pages 163-186

4. Electrophysiological Methods

   1. Front Matter

      Pages 187-187

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   2. The Use of Ion-Selective Microelectrodes to Study Cellular Transport Processes

      • J. Fernando García-Díaz, Fernando Giráldez

      Pages 189-214

   3. Voltage-Dependent Ionic Channels: “Whole-Cell” Recording by Patch-Clamp Techniques

      • José López-Barneo

      Pages 215-237

   4. Electrophysiological Methods in the Study of Water Transport Across Cell Membranes

      • Luis Reuss, Calvin U. Cotton

      Pages 239-254

5. Spectroscopic Methods

   1. Front Matter

      Pages 255-255

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   2. A Practical Introduction to the Use of Intracellular Fluorescent Indicators

      • M. Valdeolmillos, D. A. Eisner

      Pages 257-272

   3. Biomedical Nuclear Magnetic Resonance Spectroscopy and Transport

      • Susan Wray

      Pages 273-296

Experimental science is a complicated creature. At the head there is a Gordian knot of ideas and hypotheses; behind is the accumulated mass of decades of research. Only the laboratory methods, the legs which propel science forward, remain firmly in touch with the ground. Growth, however is uneven; dinosaurs develop by solid means to give a vast body of results, but few ideas. Others sprint briefly to success with brilliant, though ill-supported, ideas. The problems which this book addresses is to maintain an organic unity between new ideas and the current profusion of innovative experimental tools. Only then can we have the framework on which our research thoughts may flourish. The contributors are outstanding scientists in their respective fields and they record here in a clear manner the methodology with which they perform their experiments. They also illustrate some of their most exciting findings. In all chapters the emphasis is on the critical analysis of the methodology which is often avoided in refereed Journals. These techniques are explained in this book in adequate detail. Each chapter is extensively referenced and contains the most recent material available from author's laboratory at the time of going to press.

• ATP
• Amino acid
• Mammalia
• Nucleoside
• Tyrosin
• development
• fluorescence
• preparation
• protein
• tissue
• transport

• University of London, London, UK

  David L. Yudilevich

• University of Chile, Santiago, Chile

  Rosa Devés

• University of Málaga, Málaga, Spain

  Salvador Perán

• The Hebrew University of Jerusalem, Jerusalem, Israel

  Z. Ioav Cabantchik

Policies and ethics