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Immobilized Biochemicals and Affinity Chromatography

  • Book
  • © 1974

Overview

Editors:
  1. R. Bruce Dunlap

    1. Department of Chemistry, University of South Carolina, Columbia, USA

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Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 42)

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Table of contents (27 chapters)

  1. Front Matter

    Pages i-xi
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  2. Affinity Chromatography

    1. Front Matter

      Pages 1-1
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    2. Affinity Chromatography — Old Problems and New Approaches

      • Steven C. March, Indu Parikh, Pedro Cuatrecasas
      Pages 3-14

    3. Affinity Chromatography. New Approaches for the Preparation of Spacer Containing Derivatives and for Specific Isolation of Peptides

      • Meir Wilchek
      Pages 15-31

    4. Quantitative Parameters in Affinity Chromatography

      • A. H. Nishikawa, P. Bailon, A. H. Ramel
      Pages 33-42

    5. Non-Specific Binding of Proteins by Substituted Agaroses

      • B. H. J. Hofstee
      Pages 43-59

    6. A Solid Phase Radioimmune Assay for Ornithine Transcarbamylase

      • Donald L. Eshenbaugh, Donald Sens, Eric James
      Pages 61-73

    7. Purification of Acetylcholinesterase by Covalent Affinity Chromatography

      • Houston F. Voss, Y. Ashani, Irwin B. Wilson
      Pages 75-83

    8. Cooperative Effects of AMP, ATP and Fructose 1,6-P2 on the Specific Elution of Fructose 1,6-Diphosphatase from Cellulose Phosphate

      • Joseph Mendicino, Hussein Abou-Issa
      Pages 85-97

    9. An Analysis of Affinity Chromatography Using Immobilised Alkyl Nucleotides

      • P. D. G. Dean, D. B. Craven, M. J. Harvey, C. R. Lowe
      Pages 99-121

    10. Affinity Chromatography of Kinases and Dehydrogenases on Sephadex® and Sepharose® Dye Derivatives

      • Richard L. Easterday, Inger M. Easterday
      Pages 123-133

    11. Affinity Chromatography of Thymidylate Synthetases Using 5-Fluoro-2′-Deoxyuridine 5′-Phosphate Derivatives of Sepharose

      • John M. Whiteley, Ivanka Jerkunica, Thomas Deits
      Pages 135-146

    12. The Biosynthesis of Riboflavin: Affinity Chromatography Purification of GTP-Ring-Opening Enzyme

      • L. Preston Mercer, Charles M. Baugh
      Pages 147-155

    13. Purification of Tyrosine-Sensitive 3-Deoxy-D-Arabinoheptulosonate-7-Phosphate (DAHP) and Tyrosyl-tRNA Synthetases on Agarose Carrying Carboxyl-Linked Tyrosine

      • Andrew R. Gallopo, Philip S. Kotsiopoulos, Scott C. Mohr
      Pages 157-163

    14. Structural Requirement of Ligands for Affinity Chromatography Absorbents: Purification of Aldehyde and Xanthine Oxidases

      • Albert E. Chu, Sterling Chaykin
      Pages 165-170

  3. Immobilized Biochemicals

    1. Front Matter

      Pages 171-171
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    2. Immobilized Polynucleotides and Nucleic Acids

      • P. T. Gilham
      Pages 173-185

    3. Immobilized Cofactors and Multi-Step Enzyme-Systems

      • Klaus Mosbach
      Pages 187-189

    4. Preparation, Characterization and Applications of Enzymes Immobilized on Inorganic Supports

      • H. H. Weetall
      Pages 191-212

    5. Lactase Immobilized on Stainless Steel and Other Dense Metal and Metal Oxide Supports

      • M. Charles, R. W. Coughlin, B. R. Allen, E. K. Paruchuri, F. X. Hasselberger
      Pages 213-234
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Keywords

About this book

This volume contains most of the papers presented at the Sym­ posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi­ cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel­ lulose, glass, Sepharose, or polyacrylamide and exploits the princi­ ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish­ ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.

Editors and Affiliations

  • Department of Chemistry, University of South Carolina, Columbia, USA

    R. Bruce Dunlap

Bibliographic Information

  • Book Title: Immobilized Biochemicals and Affinity Chromatography

  • Editors: R. Bruce Dunlap

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-1-4684-6982-0

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 1974

  • Softcover ISBN: 978-1-4684-6984-4Published: 17 June 2012

  • eBook ISBN: 978-1-4684-6982-0Published: 09 March 2013

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: XI, 377

  • Topics: Biomedical Engineering and Bioengineering

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