Biophysics of the Pancreatic β-Cell

1. Front Matter

   Pages i-xii

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2. New Experimental Approaches

   1. Patch Pipettes Used for Loading Small Cells with Fluorescent Indicator Dyes

      • Erwin Neher, Wolf Almers

      Pages 1-5

   2. Optical Detection of Calcium Dependent ATP Release from Stimulated Medullary Chromaffin Cells

      • E. Rojas, E. Forsberg, H. B. Pollard

      Pages 7-29

   3. Glucose-Evoked Changes in [K+] and [Ca2+] in the Intercellular Spaces of the Mouse Islet of Langerhans

      • E. Perez-Armendariz, I. Atwater

      Pages 31-51

3. K-Channels

   1. A Potassium Channel Modulated by Glucose Metabolism in Rat Pancreatic β-Cells

      • F. M. Ashcroft, D. E. Harrison, S. J. H. Ashcroft

      Pages 53-62

   2. Glucose Suppresses ATP-Inhibited K-Channels in Pancreatic β-Cells

      • D. L. Cook, C. N. Hales, L. S. Satin

      Pages 63-67

   3. K-Channels in an Insulin-Secreting Cell Line: Effects of ATP and Sulphonylureas

      • M. L. J. Ashford, N. C. Sturgess, D. L. Cook, C. N. Hales

      Pages 69-76

   4. Inhibition of K-Channels in Insulin Secreting Cells

      • O. H. Petersen, I. Findlay, M. J. Dunne

      Pages 77-82

   5. Pharmacological Control of 86Rb Efflux from Mouse Pancreatic Islets

      • J. C. Henquin, M. G. Garrino, M. Nenquin, G. Paolisso, M. Hermans

      Pages 83-93

   6. Electrophysiological Measurements Show Marked Differences in the Properties of the Pancreatic β-Cell K-Channels from Albino Mice and a Strain of ob/ob (Obese) Mice

      • L. M. Rosario

      Pages 95-107

   7. Single K-Channel Activity in Fish Islet Cells

      • R. M. Santos, H. Finol, E. Rojas

      Pages 109-123

   8. Potassium Channels in Adrenocortical and Parathyroid Cells

      • J. Lopez-Barneo, L. Tabares, A. Castellano

      Pages 125-137

4. Ca-Channels

   1. Pharmacological Properties of the Chromaffin Cell Calcium Channel

      • A. G. Garcia, C. R. Artalejo, R. Borges, J. A. Reig, F. Sala

      Pages 139-157

   2. Insulin Release and K+-Induced Depolarization in Mouse Pancreatic β-Cells

      • S. Joost, I. Atwater

      Pages 159-166

   3. Calcium and Potassium Currents Recorded from Pancreatic β-Cells Under Voltage Clamp Control

      • G. Trube, P. Rorsman

      Pages 167-175

   4. Voltage-Activated Ca2+ and K+ Currents in an Insulin-Secreting Cell Line (RINm5F)

      • I. Findlay, M. J. Dunne

      Pages 177-187

   5. Voltage-Gated Ca Current in Pancreatic Islet β -Cells

      • L. S. Satin, D. L. Cook

      Pages 189-193

   6. Effects of Verapamil and Nifedipine on Glucose-Induced Electrical Activity in Pancreatic β-Cells

      • M. Vasseur, A. Debuyser, M. Joffre

      Pages 195-200

   7. Stimulation of Insulin Release by Organic Calcium-Agonists

      • F. Malaisse-Lagae, A. Sener, W. J. Malaisse

      Pages 201-205

5. Role of Ionic Channels

   1. Contribution of Isotope Flux Studies to Understanding the Mechanism of the β-Cell Membrane

      • P. C. Croghan, C. M. Dawson, A. M. Scott, J. A. Bangham

      Pages 207-223

Pancreatic a-cell biophysics has undergone a veritable informa­ tion explosion in the past two years. Single channel and macroscopic currents have become easily accessible following the introduction of the patch clamp technique. In addition to this new approach, further development of techniques for optical measurements, ion-sensitive microelectrodes, permeabilized cells and mathematical modelling have recently added to the now classical techniques of membrane potential recording and tracer flux measurement. The International Workshop on Biophysics of the Pancreatic a-Cell held in Alicante (Spain) on Sep­ tember 30 - October 1, 1985, has now given us the opportunity to share experiences with these new techniques applied to the a-cell. Further­ more this was the first occasion for most of the groups doing patch­ clamp studies of the a-cell to decide on appropriate nomenclature and to debate the different characteristics of the a-cell ionic channels. To make this information available to the larger scientific community a record of the meeting has been assembled in this book. It is a collection of research papers by leading scientists at the meet­ ing working on biophysical, biochemical and physiological aspects. of secretion. We grouped their contributions in seven sections, includ­ ing new experimental approaches, K-channels, Ca-channels, role of ionic channels, intracellular ionized calcium, neural regulation and mechanisms of insulin release. Each section gives an account of the state of the problem at the time of the meeting, and the subjects are analyzed from the different perspectives of the various contributors.

• Calcium
• biophysics
• cells
• membrane
• regulation

• National Institutes of Health, Bethesda, USA

  Illani Atwater, Eduardo Rojas

• University of Alicante, Alicante, Spain

  Bernat Soria

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