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Eukaryotic Cell Cultures

Basics and Applications

  • Book
  • © 1984

Overview

Editors:
  1. Ronald T. Acton

    1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. J. Daniel Lynn

    1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 172)

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Table of contents (29 chapters)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Establishing, Characterizing and Growing Cells in Culture

    1. Establishing and Characterizing Cells in Culture

      • Lewis L. Coriell
      Pages 1-11

    2. Cell Characterization by Use of Multiple Genetic Markers

      • Bharati Hukku, David M. Halton, Michael Mally, Ward D. Peterson Jr.
      Pages 13-31

    3. Characteristics of Lympho-Myelopoietic Stem Cells Isolated from Canine Peripheral Blood

      • Dennis A. Stewart, Robert B. Bolin, Barbara A. Cheney, Joseph T. Hawkins, Keith W. Chapman, Donald R. Tompkins
      Pages 33-48

    4. Growth Characteristics of A431 Human Epidermoid Carcinoma Cells in Serum-Free Medium: Inhibition by Epidermal Growth Factor

      • David W. Barnes
      Pages 49-66

    5. In Vitro Senescence, Differentiated Function, and Transformation in Cultured Vascular Endothelial Cells

      • Judith B. Grinspan, Stephen N. Mueller, James P. Noveral, Eliot M. Rosen, Elliot M. Levine
      Pages 67-90

    6. Cytogenetic Effects of Cyclamates

      • Evelyn W. Jemison, Kenneth Brown, Beverly Rivers, Regina Knight
      Pages 91-117

    7. Mammalian Cell Culture: Technology and Physiology

      • Nikos K. Harakas, Charles Lewis, Ronald D. Bartram, Bernard S. Wildi, Joseph Feder
      Pages 119-138

    8. A High Efficiency Stirrer for Suspension Cell Culture with or without Microcarriers

      • Norman A. de Bruyne
      Pages 139-149

    9. Alternative Surfaces for Microcarrier Culture of Animal Cells

      • Christine Gebb, Julian M. Clark, Michael D. Hirtenstein, Göran E. Lindgren, Bjöorn J. Lundgren, Ulla Lindskog et al.
      Pages 151-167

  3. Products of Cells in Culture

    1. Interferon Production in Microcarrier Culture of Human Fibroblast Cells

      • Victor G. Edy
      Pages 169-178

    2. In Vitro and in Vivo Effects of Endothelial Cell-Derived Growth Factor

      • Corinne M. Gajdusek, Sandra A. Harris-Hooker
      Pages 179-203

    3. Lymphotoxins — A Multicomponent System of Growth Inhibitory and Cell-Lytic Glycoproteins

      • G. A. Granger, J. Klostergaard, R. S. Yamamoto, J. Devlin, S. L. Orr, D. McGriff et al.
      Pages 205-217

    4. Production of Gamma (Immune) Interferon by a Permanent Human T-Lymphocyte Cell Line

      • Jerome E. Groopman, Ilana Nathan, David W. Golde
      Pages 219-225

    5. Thymic Inhibition of Myelopoietic Proliferation

      • Dale F. Gruber, G. David Ledney
      Pages 227-240

    6. Problems in the Bioassay of Products from Cultured HEK Cells: Plasminogen Activator

      • Marian L. Lewis, Dennis R. Morrison, Bernard J. Mieszkuc, Diane L. Fessler
      Pages 241-267

    7. A Simple Methodology for the Routine Production and Partial Purification of Human Lymphoblastoid Interferon

      • P. J. Neame, R. T. Acton
      Pages 269-279

  4. Use of Recombinant DNA Technology

    1. The Cloning, Isolation and Characterization of a Biologically Active Human Enzyme, Urokinase, in E. Coli

      • P. P. Hung
      Pages 281-293

    2. Cloning of the Rat Endogenous Helper Leukemia Virus DNA Sequence and Expression of the Helper Activity Encoded by the Cloned DNA Sequence in Normal Rat Kidney Cells by Microinjection

      • Stringer S. Yang, Rama Modali, Edwin Murphy Jr.
      Pages 295-318

    3. Analysis of CAD Gene Amplification Using a Combined Approach of Molecular Genetics and Cytogenetics

      • Geoffrey M. Wahl, Virginia Allen, Suzanne Delbruck, Walter Eckhart, Judy Meinkoth, Bruno Robert de Saint Vincent et al.
      Pages 319-345
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Keywords

About this book

The Second International Cell Culture Congress was structured as was the First Congress to bring together scientists from academia and industry to discuss the use of cell culture in support of bioscience. It was felt that a forum whereby state-of­ the-art presentations were followed by informal workshops would provide opportunity for the greatest exchange of information. Within the atmosphere of the workshop, problems common to basic as well as applied research were discussed and directions for the future were brought to light. These proceedings reflect and epitomize those discussions. Although it is difficult to cover all scientific disciplines utilizing cells in culture, we feel key areas were addressed at the Congress and are herein presented. Considerable emphasis has been given to the methods for establishing cells in culture and characterizing the cells once established as well as the improved technology for growing established cell lines. Examples of how recombinant DNA technology is being used to manipulate genes within mammalian cells, to clone mammalian genes and to insert them in prokaryotes has been included. Major emphasis has been given to the use of lymphocytes in culture for understanding immune responsiveness and the culturing of a variety of cell types as a means to understand disease states.

Editors and Affiliations

  • Department of Microbiology, University of Alabama in Birmingham, Birmingham, USA

    Ronald T. Acton, J. Daniel Lynn

Bibliographic Information

  • Book Title: Eukaryotic Cell Cultures

  • Book Subtitle: Basics and Applications

  • Editors: Ronald T. Acton, J. Daniel Lynn

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-1-4615-9376-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 1984

  • Softcover ISBN: 978-1-4615-9378-2Published: 25 November 2012

  • eBook ISBN: 978-1-4615-9376-8Published: 09 March 2013

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: X, 556

  • Topics: Biomedical Engineering and Bioengineering, Cell Biology

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