Skip to main content
SpringerLink
Log in

Methods in DNA Amplification

  • Book
  • © 1994

Overview

Editors:
  1. Arndt Rolfs

    1. Free University of Berlin, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ines Weber-Rolfs

    1. Free University of Berlin, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Ulrich Finckh

    1. Free University of Berlin, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (27 chapters)

  1. Front Matter

    Pages I-IX
    Download chapter PDF

  2. General Aspects of PCR

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Polymerase Chain Reaction and Fixed Tissues

      • John O’Leary
      Pages 3-9

    3. Impact of PCR on the Pathologist’s World

      • D. Myerson
      Pages 11-17

    4. Sensitive and Rapid Detection and Quantification of Nucleic Acids

      • L. Cross, C. Potts, J. G. Anson
      Pages 19-26

    5. Polymorphic Keratins as Detected by PCR and SSCP

      • D. Mischke, R. Wanner, B. P. Korge
      Pages 27-35

    6. Production of Antibodies of Monoclonal Specificity Without the Use of Hybridoma Cell Lines

      • T. R. Gingeras, P. Koutz, P.-J. Linton, D. J. Decker, N. R. Klinman, C. Stillman
      Pages 37-46

    7. Use of Chelex 100TM in the Extraction of Viruses from Diverse Cell-Free Clinical Samples for PCR

      • A. Ochert, M. J. Slomka, J. Ellis, C. G. Teo
      Pages 47-53

    8. Detection of Amplified Oncogenes by Differential Polymerase Chain Reaction

      • B. Brandt, U. Vogt, C. Griwatz, F. Harms, K. S. Zänker
      Pages 55-64

    9. Resolution of a Secondary Structure in an Unknown mRNA 5’ End

      • M. Bacher, P. Hofmann, D. Gemsa
      Pages 65-69

    10. Automated Solid-Phase Sequencing of Genomic DNA Obtained from Polymerase Chain Reaction

      • A. Rolfs, I. Weber
      Pages 71-79

  3. Alternative DNA-Amplification Methods

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. Ligase-Mediated Detection Techniques

      • Martin Wiedmann, Wendy Wilson, John Czajka, Francis Barany, Carl A. Batt
      Pages 83-92

    3. Qualitative and Quantitative Detection of Nucleic Acids of Infectious Agents by NASBA

      • B. van Gemen, T. Kievits, P. F. Lens
      Pages 93-102

    4. Application of NASBA to the Detection of Listeria Monocytogenes

      • J. Kleiber, C. Kaletta, M. Hartl, C. Kessler, P. Kirch, C. Majewski
      Pages 103-110

  4. PCR and Virological Problems

    1. Front Matter

      Pages 111-111
      Download chapter PDF

    2. Confirmation of Hepatitis C Virus Carrier State by Polymerase Chain Reaction in Cases with Nondiagnostic Serologies

      • Ch.-H. Wang, B. Flehmig
      Pages 113-121

    3. Confirmation of Hepatitis C Virus Positive Seras by Polymerase Chain Reaction

      • G. Lucotte, C. Mura, A. Aouizenate, T. Champenois, J. Marchand
      Pages 123-126

    4. Specific Detection and Rapid Identification of Human Enteroviruses by PCR Amplification

      • U. Kämmerer, B. Kunkel, K. Korn
      Pages 127-134

    5. Detection of Herpes Simplex Virus Using Polymerase Chain Reaction

      • H. H. Kessler, K. Pierer, D. Stünzner, E. Marth
      Pages 135-143
Back to top

Keywords

About this book

The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR­ result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure.

Editors and Affiliations

  • Free University of Berlin, Berlin, Germany

    Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh

Bibliographic Information

  • Book Title: Methods in DNA Amplification

  • Editors: Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh

  • DOI: https://doi.org/10.1007/978-1-4615-2530-1

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1994

  • Hardcover ISBN: 978-0-306-44908-6Published: 31 December 1994

  • Softcover ISBN: 978-1-4613-6078-0Published: 07 November 2012

  • eBook ISBN: 978-1-4615-2530-1Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: IX, 251

  • Topics: Infectious Diseases, Cell Biology, Animal Anatomy / Morphology / Histology, Plant Sciences

Publish with us

Policies and ethics

Back to top

Search

Navigation