Genetic Engineering

1. Front Matter

   Pages i-xvi

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2. Folding of Eukaryotic Proteins Produced in Escherichia Coli

   • Robert F. Kelley, Marjorie E. Winkler

   Pages 1-19

3. Human Retinoblastoma Susceptibility Gene

   • Chen-Ching Lai, Wen-Hwa Lee

   Pages 21-35

4. α-Oligodeoxynucleotides (α-DNA): A New Chimeric Nucleic Acid Analog

   • F. Morvan, B. Rayner, J.-L. Imbach

   Pages 37-52

5. The Utility of Streptomycetes as Hosts for Gene Cloning

   • Paul K. Tomich, Yoshihiko Yagi

   Pages 53-72

6. From Footprint to Function: An Approach to Study Gene Expression and Regulatory Factors in Transgenic Plants

   • Eric Lam

   Pages 73-86

7. Purification of Recombinant Proteins with Metal Chelate Adsorbent

   • Erich Hochuli

   Pages 87-98

8. Determinants of Translation Efficiency of Specific mRNAs in Mammalian Cells

   • David S. Peabody

   Pages 99-113

9. The Polymerase Chain Reaction

   • Norman Arnheim

   Pages 115-137

10. Regulation of Alternative Splicing

    • Michael McKeown

    Pages 139-181

11. Structure and Function of the Nuclear Receptor Superfamily for Steroid, Thyroid Hormone and Retinoic Acid

    • Vincent Giguère

    Pages 183-200

12. Identification and Functional Analysis of Mammalian Splicing Factors

    • Albrecht Bindereif, Michael R. Green

    Pages 201-224

13. The Genes Encoding Wheat Storage Proteins: Towards a Molecular Understanding of Bread-Making Quality and Its Genetic Manipulation

    • Vincent Colot

    Pages 225-242

14. Control of Translation Initiation in Hamnalian Cells

    • Randal J. Kaufman

    Pages 243-273

15. Electroporation of Bacteria: A General Approach to Genetic Transformation

    • William J. Dower

    Pages 275-295

16. The Isolation and Identification of cDNA Genes by Their Heterologous Expression and Function

    • Gordon G. Wong

    Pages 297-316

17. Molecular Cloning of Genes Encoding Transcription Factors with the Use of Recognition Site Probes

    • Harinder Singh

    Pages 317-330

18. Back Matter

    Pages 331-339

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• Retinoblastom
• Translation
• cloning
• gene expression
• genes
• genetic engineering
• genetic transformation
• recombinant proteins
• transcription
• wheat
• Tree Biology

• Brookhaven National Laboratory, Upton, USA

  Jane K. Setlow

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

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