Flow Cytometry in Microbiology

1. Front Matter

   Pages i-xi

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2. Flow Cytometry: A Technique Waiting for Microbiologists

   • David Lloyd

   Pages 1-9

3. The Physical and Biological Basis for Flow Cytometry of Escherichia coli

   • Erik Boye, Harald B. Steen

   Pages 11-25

4. Flow Cytometric Analysis of Heterogeneous Bacterial Populations

   • Richard Allman, Richard Manchee, David Lloyd

   Pages 27-47

5. On the Determination of the Size of Microbial Cells Using Flow Cytometry

   • Hazel M. Davey, Chris L. Davey, Douglas B. Kell

   Pages 49-65

6. Uses of Membrane Potential Sensitive Dyes with Bacteria

   • David Mason, Richard Allman, David Lloyd

   Pages 67-81

7. Flow Cytometric Analysis, Using Rhodamine 123, of Micrococcus luteus at Low Growth Rate in Chemostat Culture

   • Hazel M. Davey, Arseny S. Kaprelyants, Douglas B. Kell

   Pages 83-93

8. Characterization of Bacterial Cell Size and Ploidy using Flow Cytometry and Image Analysis

   • Jerome Durodie, Kenneth Coleman, Michael J. Wilkinson

   Pages 95-109

9. Cytometric Evaluation of Antifungal Agents

   • Elizabeth A. Carter, Frank E. Paul, Pamela A. Hunter

   Pages 111-120

10. Applications of Flow Cytometry in Bacterial Ecology

    • Clive Edwards, Julian P. Diaper, Jonathan Porter, Roger Pickup

    Pages 121-129

11. Analysis of Microalgae and Cyanobacteria by Flow Cytometry

    • Alex Cunningham

    Pages 131-142

12. Flow Cytometry at Sea

    • Glen A. Tarran, Peter H. Burkill

    Pages 143-158

13. Neural Network Analysis of Flow Cytometry Data

    • Lynne Boddy, Colin W. Morris

    Pages 159-169

14. Rapid Analysis of Microorganisms Using Flow Cytometry

    • Michael Brailsford, Stephen Gatley

    Pages 171-180

15. Appendix: Spectral Characteristics of Some Fluorescent Dyes and Excitation Sources

    • David Lloyd

    Pages 181-183

16. Back Matter

    Pages 185-188

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As yet, flow cytometry is not used so widely in microbiology as in some other disciplines. This volume presents contributions flow cytometry to study a from research microbiologists who use diverse set of problems. It illustrates the power of the technique, and may persuade others of its usefulness. Most of the con­ tributors gathered in Cardiff on 23 October 1991, at a meeting organized for the Royal Microscopical Society by Dr. Richard Allman, but the content of their chapters is not limited by the discourse of that meeting, and for balance other experts were invited to write for this book. Flow Cytometry in Microbiology thus represents the first collection of articles specifically devoted to the applications of a technique which promises so much to those investigating the microbial world. Cardiff, 1992 David Lloyd Contents List of Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Flow Cytometry: A Technique Waiting for Microbiologists David Lloyd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 The Physical and Biological Basis for Flow Cytometry of Escherichia coli Erik Boye and Harald B. Steen . . . . . . . . . . . . . . . . . . . . . . 11 3 Flow Cytometric Analysis of Heterogeneous Bacterial Populations Richard Allman, Richard Manchee and David Lloyd. . . . 27 4 On the Determination of the Size of Microbial Cells Using Flow Cytometry Hazel M. Davey, Chris L. Davey and Douglas B. Kell . . 49 5 Uses of Membrane Potential Sensitive Dyes with Bacteria David Mason, Richard Allman and David Lloyd . . . . . . .

• Micro-organisms
• Protozoa
• Yeasts
• antibiotic
• bacteria
• biology
• cell
• ecology
• escherichia coli
• flow cytometry
• membrane
• metabolism
• micro-organism
• microbiology
• microorganism

• University of Wales College of Cardiff, Cardiff, Wales

  David Lloyd

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