Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis

1. Front Matter

   Pages i-xiv

   PDF

2. Introduction

   • George G. Harrigan, Royston Goodacre

   Pages 1-8

3. Metabolome and Proteome Profiling for Microbial Characterization

   • Seetharaman Vaidyanathan, Royston Goodacre

   Pages 9-38

4. Can 1H NMR Derived Metabolic Profiles Contribute to Proteomic Analyses?

   • Julian L. Griffin, Elizabeth Sang

   Pages 39-68

5. Application of Metabonomics in the Pharmaceutical Industry

   • Alan P. Breau, Glenn H. Cantor

   Pages 69-82

6. Metabolic Profiling in Tumors by In Vivo and In Vitro NMR Spectroscopy

   • Yeun-Li Chung, Marion Stubbs, John R. Griffiths

   Pages 83-94

7. Raman Spectroscopy for Whole Organism and Tissue Profiling

   • Sarah Clarke, Royston Goodacre

   Pages 95-110

8. Metabolic Fingerprinting with Fourier Transform Infrared Spectroscopy

   • David I. Ellis, George G. Harrigan, Royston Goodacre

   Pages 111-124

9. Metabolomic Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   • Dayan Goodenowe

   Pages 125-139

10. Stable Isotope-Based Dynamic Metabolic Profiling in Disease and Health

    • László G. Boros, Marta Cascante, Wai-Nang Paul Lee

    Pages 141-169

11. The Role of Metabolomics in Systems Biology

    • Jan van der Greef, Eugene Davidov, Elwin Verheij, Jack Vogels, Rob van der Heijden, Aram S. Adourian et al.

    Pages 171-198

12. Use of Metabolomics to Discover Metabolic Patterns Associated with Human Diseases

    • Oliver Fiehn, Joachim Spranger

    Pages 199-215

13. Bioactive Lipids in Reproductive Diseases

    • Jaideep Chaudhary, Tim Compton, Jeff A. Parrott

    Pages 217-238

14. Evolutionary Computation for the Interpretation of Metabolomic Data

    • Royston Goodacre, Douglas B. Kell

    Pages 239-256

15. Dynamic Profiling and Canonical Modeling

    • Eberhard O. Voit, Jonas Almeida

    Pages 257-276

16. Databases, Data Modeling and Schemas

    • Nigel Hardy, Helen Fuell

    Pages 277-291

17. Databases and Visualization for Metabolomics

    • X. Jing Li, Olga Brazhnik, Aejaaz Kamal, Dianjing Guo, Christine Lee, Stefan Hoops et al.

    Pages 293-309

18. The Human Metabolome

    • Chris W. W. Beecher

    Pages 311-319

19. Back Matter

    Pages 321-335

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It is evident that biochemical control is not strictly hierarchical and that intermediary metabolism can contribute to control of regulatory pathways. Metabolic studies are therefore increasingly important in gene function analyses, and an increased interest in metabolites as biomarkers for disease progression or response to therapeutic intervention is also evident in the pharmaceutical industry. This book offers guidelines to currently available technology and bioinformatics and database strategies now being developed. Evidence is presented that metabolic profiling is a valuable addition to genomics and proteomics strategies devoted to drug discovery and development, and that metabolic profiling offers numerous advantages.

• Proteomics
• biology
• metabolism
• nuclear magnetic resonance (NMR)
• spectroscopy

• Pharmacia Corporation, Chesterfield, USA

  George G. Harrigan

• Department of Chemistry, UMIST, Manchester, UK

  Royston Goodacre

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