Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2148)
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Table of contents (25 protocols)
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Front Matter
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Methods for Cultured Cells
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Front Matter
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Methods for Wholemounts and Plant Material
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Front Matter
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Automated Methods for RNA
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Front Matter
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About this book
This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field.
Editors and Affiliations
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Molecular Histology, Bioneer A/S, Horsholm, Denmark
Boye Schnack Nielsen
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Cancer Research UK, University of Cambridge, Cambridge, UK
Julia Jones
Bibliographic Information
Book Title: In Situ Hybridization Protocols
Editors: Boye Schnack Nielsen, Julia Jones
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0623-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0622-3Published: 12 May 2020
Softcover ISBN: 978-1-0716-0625-4Published: 12 May 2021
eBook ISBN: 978-1-0716-0623-0Published: 11 May 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 5
Number of Pages: XIII, 392
Number of Illustrations: 1 b/w illustrations, 90 illustrations in colour
Topics: Genetics and Genomics