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CRISPR-Cas Methods

  • Book
  • © 2020

Overview

Editors:
  1. M. Tofazzal Islam

    1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

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  2. Pankaj K. Bhowmik

    1. Department of Aquatic and Crop Resource Development, National Research Council of Canada, SASKATOON, Canada

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  3. Kutubuddin A. Molla

    1. Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, India

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine

    • Kutubuddin A. Molla, Subhasis Karmakar, M. Tofazzal Islam
    Pages 1-23

  3. Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis

    • Felix Wolter, Teng-Kuei Huang, Holger Puchta
    Pages 25-34

  4. Rice Gene Knockout or Downregulation through CRISPR-Cas9

    • Yvonne Ludwig, Inez H. Slamet-Loedin
    Pages 35-50

  5. CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome

    • Kutubuddin A. Molla, Yinong Yang
    Pages 51-62

  6. CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species Brachypodium distachyon

    • Deborah Petrik
    Pages 63-86

  7. CRISPR-Cas9-Mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora

    • Miaoying Tian, Natasha Navet, Dongliang Wu
    Pages 87-98

  8. An Effective CRISPR-Cas9 Technology for Efficiently Isolating Transgene-Free Mutants in Arabidopsis, Brassica napus, Strawberry, and Soybean

    • Cheng Dai, Hong Yang, Ting Tang, Chaozhi Ma, Chunying Kang
    Pages 99-115

  9. An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (Hordeum vulgare L.)

    • Sebastian Gasparis, Mateusz Przyborowski
    Pages 117-142

  10. Genome Editing of Mammalian Cells Using CRISPR-Cas: From In Silico Designing to In-Culture Validation

    • Borhan Uddin, Patrick Partscht, Taslima Nahar
    Pages 143-162

  11. Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detection

    • Magdalena Åšmiech, PaweÅ‚ LeszczyÅ„ski, Effi Haque, Hiroaki Taniguchi
    Pages 163-171

  12. A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome Editing in Flax

    • Dinesh Adhikary, Isadora Louise Alves da Costa Ribeiro Quintans, Pankaj K. Bhowmik
    Pages 173-190

  13. Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Expression Modulation

    • Andriy Bilichak, Justin Luu, Fengying Jiang, John Laurie
    Pages 191-202

  14. CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol

    • Pankaj K. Bhowmik, M. Tofazzal Islam
    Pages 203-222

  15. CRISPR-Cas9-Based Genome Editing of Banana

    • Leena Tripathi, Valentine Otang Ntui, Jaindra Nath Tripathi
    Pages 223-236

  16. Homology-Directed Transgene-Free Gene Editing in Chlamydomonas reinhardtii

    • Aron Ferenczi, Attila Molnar
    Pages 237-252

  17. Correction to: A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome Editing in Flax

    • Dinesh Adhikary, Isadora Louise Alves da Costa Ribeiro Quintans, Pankaj K. Bhowmik
    Pages C1-C1

  18. Back Matter

    Pages 253-278
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Keywords

About this book

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 


Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Editors and Affiliations

  • Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

  • Department of Aquatic and Crop Resource Development, National Research Council of Canada, SASKATOON, Canada

    Pankaj K. Bhowmik

  • Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, India

    Kutubuddin A. Molla

About the editors


Bibliographic Information

  • Book Title: CRISPR-Cas Methods

  • Editors: M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-0616-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0615-5Published: 30 June 2020

  • Softcover ISBN: 978-1-0716-0618-6Published: 05 July 2020

  • eBook ISBN: 978-1-0716-0616-2Published: 30 June 2020

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XIV, 278

  • Number of Illustrations: 9 b/w illustrations, 58 illustrations in colour

  • Topics: Genetics and Genomics

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