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Lectin Purification and Analysis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2132)

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Table of contents (60 protocols)

  1. Front Matter

    Pages i-xviii
  2. Structural Database for Lectins and the UniLectin Web Platform

    • François Bonnardel, Serge Perez, Frédérique Lisacek, Anne Imberty
    Pages 1-14
  3. Identification of Siglec Cis-Ligands by Proximity Labeling

    • Amin Alborzian Deh Sheikh, Chizuru Akatsu, Takeshi Tsubata
    Pages 75-83
  4. Preparation of Recombinant Siglecs and Identification of Their Ligands

    • Lan-Yi Chang, Penk Yeir Low, Deepa Sridharan, Kaia Gerlovin, Takashi Angata
    Pages 85-98
  5. Purification, Quantification, and Functional Analysis of Collectins

    • Katsuki Ohtani, Nobutaka Wakamiya
    Pages 99-109
  6. Selectin-Binding Assay by Flow Cytometry

    • Keiichiro Sakuma, Reiji Kannagi
    Pages 111-118
  7. Paracoccin: Purification and Validation of Its Lectin and Enzymatic Properties

    • Nayla de Souza Pitangui, Fabrício Freitas Fernandes, Relber Aguiar Gonçales, Maria Cristina Roque-Barreira
    Pages 139-149
  8. Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay

    • Kyoka Hoshi, Yasuhiro Hashimoto, Hiromi Ito
    Pages 165-171
  9. Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition”

    • Hiromi Ito, Kyoka Hoshi, Yasuhiro Hashimoto, Takashi Honda
    Pages 173-181

About this book

This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.



Editors and Affiliations

  • Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

Bibliographic Information

  • Book Title: Lectin Purification and Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Jun Hirabayashi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0430-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0429-8Published: 18 April 2020

  • Softcover ISBN: 978-1-0716-0432-8Published: 18 April 2021

  • eBook ISBN: 978-1-0716-0430-4Published: 18 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 699

  • Number of Illustrations: 82 b/w illustrations, 110 illustrations in colour

  • Topics: Protein Science, Plant Sciences

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 239.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access