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Innate Lymphoid Cells

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Shoba Amarnath

    1. Laboratory of T cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2121)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Protocols for Innate Lymphoid Cell Phenotypic and Functional Characterization: An Overview

    • Shoba Amarnath
    Pages 1-6

  3. Protocols for Studying Murine ILC Development

    • Matthew T. Stier, R. Stokes Peebles Jr
    Pages 7-22

  4. Identification of Murine Innate Lymphoid Cell Subsets in Barrier Tissues and Their Draining Lymph Nodes Using Flow Cytometry

    • Emma E. Dutton, David R. Withers
    Pages 23-36

  5. Identification and Functional Characterization of Murine Group 3 Innate Lymphoid Cell (ILC3) Subsets in the Intestinal Tract and Associated Lymphoid Tissues

    • Felipe Melo-Gonzalez, Matthew R. Hepworth
    Pages 37-49

  6. Identification of Murine and Human Innate Lymphoid Cells in Frozen Tissue Sections Using Immunofluorescence

    • Emma E. Dutton, David R. Withers
    Pages 51-58

  7. Assessing Phosphorylation of STAT Transcription Factors in Mouse Innate Lymphoid Cells

    • Gianluca Scarno, Giuseppe Pietropaolo, Chiara Di Censo, Giovanna Peruzzi, Giuseppe Sciumè
    Pages 59-70

  8. Determining Coreceptor Expression and Function in Murine ILC2 Through Flow Cytometry Characterization and Coculture Techniques

    • Christian Schwartz, Padraic G. Fallon
    Pages 71-82

  9. Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function

    • Linda Quatrini, Sophie Ugolini
    Pages 83-92

  10. Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells

    • Brinda Bhatt, Huabin Zhu, Nikhil Patel, Nagendra Singh
    Pages 93-98

  11. Group 2 ILC Functional Assays in Allergic Airway Inflammation

    • Lewis J. Entwistle, Franz Puttur, Lisa G. Gregory, Clare M. Lloyd
    Pages 99-114

  12. Isolation, Culture, and Induction of Plasticity in ILC2s

    • Jonathan Silver, Alison A. Humbles, Yoichiro Ohne
    Pages 115-127

  13. Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets

    • Goo-Young Seo, Daniel A. Giles, Mitchell Kronenberg
    Pages 129-140

  14. Isolation, Characterization, and Culture of Intestinal Intraepithelial Lymphocytes

    • Olivia Jane James, Maud Vandereyken, Mahima Swamy
    Pages 141-152

  15. Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

    • Grace Mallett, Warren Patterson, Megan Payne, Shoba Amarnath
    Pages 153-164

  16. Microbiota Analysis Using Sequencing by Synthesis: From Library Preparation to Sequencing

    • Andrew Nelson, Christopher J. Stewart
    Pages 165-184

  17. Establishing Human Intestinal Enteroid/Organoid Lines from Preterm Infant and Adult Tissue

    • Christopher J. Stewart, Mary K. Estes, Sasirekha Ramani
    Pages 185-198

  18. Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease

    • Omer S. Omer, Nick Powell, Graham M. Lord
    Pages 199-211

  19. CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

    • Mélanie Lambert, Caroline Leijonhufvud, Filip Segerberg, J. Joseph Melenhorst, Mattias Carlsten
    Pages 213-239

  20. Back Matter

    Pages 241-242
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Keywords

About this book

This volume explores the latest techniques used to characterize murine and human Innate Lymphoid Cells (ILC) subsets. This book also aims to discuss useful phenotypical and functional assays that may be relevant for studying ILC function in mice and humans. Chapters cover topics such as methods to study microbiome and enteroid cultures; characterizing ILC precursors; and cellular engineering of ILCs for therapeutic use. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Innate Lymphoid Cells: Methods and Protocols is a valuable research tool for anyone interested in ILC biology, and those interested in fundamental immunology, cellular engineering, cell signaling, and clinician scientists. 



Editors and Affiliations

  • Laboratory of T cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, UK

    Shoba Amarnath

Bibliographic Information

  • Book Title: Innate Lymphoid Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Shoba Amarnath

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0338-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0337-6Published: 09 March 2020

  • Softcover ISBN: 978-1-0716-0340-6Published: 09 March 2021

  • eBook ISBN: 978-1-0716-0338-3Published: 08 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 242

  • Number of Illustrations: 9 b/w illustrations, 39 illustrations in colour

  • Topics: Cancer Research, Immunology, Cell Biology

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