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Epidermal Cells

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2109)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
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  2. The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse

    • Filipa Pinto, Daisuke Suzuki, Makoto Senoo
    Pages 1-22

  3. Dermal-Epidermal Separation by Heat

    • Liyan Jian, Yu Cao, Ying Zou
    Pages 23-25

  4. Dermal-Epidermal Separation by Enzyme

    • Liyan Jian, Yu Cao, Ying Zou
    Pages 27-30

  5. Dermal-Epidermal Separation by Chemical

    • Liyan Jian, Yu Cao, Ying Zou
    Pages 31-33

  6. Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

    • Sebastian Sjöqvist, Aya Imafuku, Dhanu Gupta, Samir EL Andaloussi
    Pages 35-44

  7. Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential

    • Carmen Segrelles, Karla Santos-de-Frutos, Jesús M. Paramio, Corina Lorz
    Pages 45-53

  8. Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

    • Aishwarya Sridharan, S. Y. John Lim, Graham D. Wright, Leah A. Vardy
    Pages 55-65

  9. Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

    • Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, Massimo Libra, Fabio D’Amico
    Pages 67-74

  10. Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry

    • Manuel Valdebran, Eric H. Kowalski, Diana Kneiber, Jing Li, Jeffrey Kim, Kyle T. Amber
    Pages 75-82

  11. Keratinocyte Differentiation by Flow Cytometry

    • Natalia Sanz-Gómez, Ana Freije, Alberto Gandarillas
    Pages 83-92

  12. In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis

    • Rambon Shamilov, Matthew J. Staid, Brian J. Aneskievich
    Pages 93-111

  13. Genetic Modification of Human Primary Keratinocytes by Lentiviral Vectors

    • Ana Freije, Natalia Sanz-Gómez, Alberto Gandarillas
    Pages 113-123

  14. Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9

    • Serena Grossi, Gabriele Fenini, Paulina Hennig, Michela Di Filippo, Hans-Dietmar Beer
    Pages 125-145

  15. In Vitro Wound Healing Assays to Investigate Epidermal Migration

    • Kegan A. Main, Constantinos M. Mikelis, Colleen L. Doçi
    Pages 147-154

  16. Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells

    • Nicolas O. Fortunel, Emmanuelle Bouissou-Cadio, Julien Coutier, Michèle T. Martin
    Pages 155-167

  17. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse

    • Nicole Diette, Igor Kogut, Ganna Bilousova
    Pages 169-183

  18. Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures

    • Calvin J. Cable, Nihal Kaplan, Spiro Getsios, Paul M. Thomas, Bethany E. Perez White
    Pages 185-197

  19. Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data

    • Shinji Nakaoka, Keita Matsuyama
    Pages 199-208

  20. Cell-Extracellular Matrix Adhesion Assay

    • Mehmet Varol
    Pages 209-217
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Keywords

About this book

This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function. The collection takes a detailed view at techniques currently being applied to this developing field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Epidermal Cells: Methods and Protocols, Fourth Edition serves as a valuable reference for experts as well as novices in the epidermal cell field.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Epidermal Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0251-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0250-8Published: 15 March 2020

  • Softcover ISBN: 978-1-0716-0253-9Published: 14 March 2020

  • eBook ISBN: 978-1-0716-0251-5Published: 14 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XII, 301

  • Number of Illustrations: 14 b/w illustrations, 43 illustrations in colour

  • Topics: Cell Biology

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