Biopolymer Methods in Tissue Engineering

1. Front Matter

   Pages i-xiv

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2. Processing of Resorbable Poly-α-Hydroxy Acids for Use as Tissue-Engineering Scaffolds

   • Minna Kellomäki, Pertti Törmälä

   Pages 1-10

3. Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing

   • Raphael Gorodetsky, Akiva Vexler, Lilia Levdansky, Gerard Marx

   Pages 11-24

4. Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering

   • Carlo Soranzo, Davide Renier, Alessandra Pavesio

   Pages 25-39

5. Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering

   • Steven H. Elder, Dana L. Nettles, Joel D. Bumgardner

   Pages 41-48

6. Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2

   • Hyun D. Kim, John M. Wozney, Rebecca H. Li

   Pages 49-64

7. Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications

   • Manuela E. Gomes, Patrícia B. Malafaya, Rui L. Reis

   Pages 65-75

8. Alginates in Tissue Engineering

   • Marcy Wong

   Pages 77-86

9. Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering

   • Yang Cao, Tristan I. Croll, Justin J. Cooper-White, Andrea J. O’ Connor, Geoffrey W. Stevens

   Pages 87-111

10. Modification of Materials With Bioactive Peptides

    • Jennifer L. West

    Pages 113-121

11. Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering

    • AntÓnio J. Salgado, Manuela E. Gomes, Olga P. Coutinho, Rui L. Reis

    Pages 123-129

12. Cell Seeding of Polymer Scaffolds

    • Gordana Vunjak-Novakovic, Milica Radisic

    Pages 131-145

13. Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds

    • Aileen Crawford, Sally C. Dickinson

    Pages 147-157

14. Bioreactor Culture Techniques for Cartilage-Tissue Engineering

    • David A. Lee, Ivan Martin

    Pages 159-170

15. Microscopic Methods for the Analysis of Engineered Tissues

    • Sally Roberts, Janis Menage

    Pages 171-195

16. Transmission Electron Microscopy of Tissue-Polymer Constructs

    • Paul V. Hatton

    Pages 197-206

17. Application of Microscopic Methods for the Detection of Cell Attachment to Polymers

    • John Hunt, Deborah Heggarty

    Pages 207-216

18. Biochemical Methods for the Analysis of Tissue-Engineered Cartilage

    • Wáel Kafienah, Trevor J. Sims

    Pages 217-229

19. Real-Time Quantitative RT-PCR Assays

    • Ivan Martin, Oliver Frank

    Pages 231-237

20. Mechanical Testing of Cell-Material Constructs: A Review

    • John Kisiday, Alex Kerin, Alan Grodzinsky

    Pages 239-254

There is an urgent need to develop new approaches to treat conditions as- ciated with the aging global population. The surgeon’s approach to many of these problems could be described as having evolved through three stages: Removal: Traditionally, diseased or badly damaged tissues and structures might simply be removed. This was appropriate for limbs and non-essential organs, but could not be applied to structures that were critical to sustain life. An additional problem was the creation of disability or physical deformity that in turn could lead to further complications. Replacement: In an effort to treat wider clinical problems, or to overcome the limitations of amputation, surgeons turned to the use of implanted materials and medical devices that could replace the functions of biological structures. This field developed rapidly in the 1960s and 1970s, with heart valve and total joint replacement becoming common. The term “biomaterial” was used increasingly to describe the materials used in these operations, and the study of biomaterials became one of the first truly interdisciplinary research fields. Today, biomaterials are employed in many millions of clinical procedures each year and they have become the mainstay of a very successful industry.

From the reviews:

"This small, 280-page volume is useful to people who are interested in using various laboratory techniques to conduct tissue engineering research. … Tips on troubleshooting and avoiding pitfalls which appear at the end of nearly all chapters will be welcomed by users of the book. The goal of this book is to ‘serve as a basic laboratory manual allowing tissue engineering scientists not only to access a wide range of techniques in one place, but also to have them described using a standard format’." (Annales de Chimie Science des Materiaux, Vol. 29 (4), 2004)

• Centre for Biomaterials and Tissue Engineering, School of Clinical Dentistry, University of Sheffield, Sheffield, UK

  Anthony P. Hollander

• Avon Orthopaedic Centre, Southmead Hospital, University of Bristol Academic Rheumatology, Bristol, UK

  Paul V. Hatton

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