Buffers for pH and Metal Ion Control

1. Front Matter

   Pages i-viii

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2. Introduction

   • D. D. Perrin, Boyd Dempsey

   Pages 1-3

3. The Theory of Buffer Action

   • D. D. Perrin, Boyd Dempsey

   Pages 4-23

4. Applications of pH Buffers

   • D. D. Perrin, Boyd Dempsey

   Pages 24-54

5. Practical Limitations in the use of Buffers

   • D. D. Perrin, Boyd Dempsey

   Pages 55-61

6. New pH-Buffer Tables and Systems

   • D. D. Perrin, Boyd Dempsey

   Pages 62-76

7. Buffers for use in Partially Aqueous and Non-Aqueous Solvents and Heavy Water

   • D. D. Perrin, Boyd Dempsey

   Pages 77-93

8. Metal-Ion Buffers

   • D. D. Perrin, Boyd Dempsey

   Pages 94-108

9. Purification of Substances used in Buffers

   • D. D. Perrin, Boyd Dempsey

   Pages 109-116

10. Preparation of Buffer Solutions

    • D. D. Perrin, Boyd Dempsey

    Pages 117-122

11. Appendices

    • D. D. Perrin, Boyd Dempsey

    Pages 123-166

12. Back Matter

    Pages 167-176

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This book is intended as a practical manual for chemists, biologists and others whose work requires the use of pH or metal-ion buffers. Much information on buffers is scattered throughout the literature and it has been our endeavour to select data and instructions likely to be helpful in the choice of suitable buffer substances and for the preparation of appropriate solutions. For details of pH measurement and the preparation of standard acid and alkali solutions the reader is referred to a companion volume, A. Albert and E. P. Serjeant's The Determination of Ionization Constants (1971). Although the aims of the book are essentially practical, it also deals in some detail with those theoretical aspects considered most helpful to an understanding of buffer applications. We have cast our net widely to include pH buffers for particular purposes and for measurements in non-aqueous and mixed solvent systems. In recent years there has been a significant expansion in the range of available buffers, particularly for biological studies, largely in conse­ quence of the development of many zwiUerionic buffers by Good et al. (1966). These are described in Chapter 3.

• Ampholyte
• Chromat
• Titration
• amines
• biochemistry
• chemical reaction
• chromatography
• electrolyte
• electrophoresis
• extraction
• metals
• purification
• spectroscopy

• John Curtin School of Medical Research, Australian National University, Canberra, Australia

  D. D. Perrin

• Faculty of Military, Studies University of New South Wales Royal Military College, Duntroon, Australia

  Boyd Dempsey

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