Optogenetics

1. Front Matter

   Pages i-xii

   PDF

2. Light-Sensitive Channels and Transporters

   1. Front Matter

      Pages 1-1

      PDF

   2. History and Perspectives of Ion-Transporting Rhodopsins

      • Hideki Kandori

      Pages 3-19

   3. Channelrhodopsin-Dependent Photo-Behavioral Responses in the Unicellular Green Alga Chlamydomonas reinhardtii

      • Ken-ichi Wakabayashi, Atsuko Isu, Noriko Ueki

      Pages 21-33

   4. Structure–Function Relationship of Channelrhodopsins

      • Hideaki E. Kato

      Pages 35-53

   5. Functional Mechanism of Cl−-Pump Rhodopsin and Its Conversion into H+ Pump

      • Takashi Kikukawa

      Pages 55-71

   6. Optogenetic Modulation of Ion Channels by Photoreceptive Proteins

      • Hisao Tsukamoto, Yuji Furutani

      Pages 73-88

   7. Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins

      • Keiichi Inoue

      Pages 89-126

3. Light-Sensitive Signaling Molecules

   1. Front Matter

      Pages 127-127

      PDF

   2. Photoactivated Adenylyl Cyclases: Fundamental Properties and Applications

      • Mineo Iseki, Sam-Yong Park

      Pages 129-139

   3. Optogenetic Potentials of Diverse Animal Opsins: Parapinopsin, Peropsin, LWS Bistable Opsin

      • Mitsumasa Koyanagi, Tomoka Saito, Seiji Wada, Takashi Nagata, Emi Kawano-Yamashita, Akihisa Terakita

      Pages 141-151

   4. Molecular Properties and Optogenetic Applications of Enzymerhodopsins

      • Satoshi P. Tsunoda, Masahiro Sugiura, Hideki Kandori

      Pages 153-165

   5. Phytochromes and Cyanobacteriochromes: Photoreceptor Molecules Incorporating a Linear Tetrapyrrole Chromophore

      • Keiji Fushimi, Rei Narikawa

      Pages 167-187

   6. Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications

      • Tatsuya Iwata, Shinji Masuda

      Pages 189-206

4. Optogenetics in Biological Systems

   1. Front Matter

      Pages 207-207

      PDF

   2. Genetically Encoded Voltage Indicators

      • Irene Mollinedo-Gajate, Chenchen Song, Thomas Knöpfel

      Pages 209-224

   3. Visualization and Manipulation of Intracellular Signaling

      • Yuhei Goto, Yohei Kondo, Kazuhiro Aoki

      Pages 225-234

   4. Light Control of Gene Expression Dynamics

      • Akihiro Isomura

      Pages 235-246

   5. Functional Modulation of Receptor Proteins on Cellular Interface with Optogenetic System

      • Mizuki Endo, Takeaki Ozawa

      Pages 247-263

   6. Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function

      • Yemima Dani Riani, Tomoki Matsuda, Takeharu Nagai

      Pages 265-279

This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular functions can thus be manipulated or probed by light. The new edition documents fully the extensive progress since publication of the first edition to provide an up-to-date overview of the physical, chemical, and biological properties of light-sensing proteins and their application in biological systems, particularly in neuroscience but also in medicine and the optical sciences. Underlying principles are explained and detailed information provided on a wide rangeof optogenetic tools for the observation and control of cellular signaling and physiology, gene targeting technologies, and optical methods for biological applications. In presenting the current status of optogenetics and emerging directions, this milestone publication will be a “must read” for all involved in research in any way related to optogenetics.

• Channelrhodopsin
• Halorhodopsin
• Neural circuits
• Optical sensors
• Psychiatry
• Visual prosthesis
• Optogenetics
• rhodopsin
• Biological light sensor
• Neuroscience
• neurobiology
• cell biology
• optoelectronics

• The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan

  Hiromu Yawo

• OptoBioTechnology Research Center, Nagoya Institute of Technology, Nagoya, Japan

  Hideki Kandori

• National Institutes of Natural Sciences, Tokyo, Japan

  Amane Koizumi

• Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan

  Ryoichiro Kageyama

Hiromu Yawo MD PhD

Tohoku University
 
Hideki Kandori PhD
Nagoya Institute of Technology
 
Amane Koizumi PhD
National Institutes of Natural Sciences
 
Ryoichiro Kageyama PhD
Kyoto University, Institute for Frontier Life and Medical Sciences

Policies and ethics