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Synthesis of Therapeutic Oligonucleotides

  • Book
  • © 2018

Overview

Editors:
  1. Satoshi Obika

    1. Osaka University, Suita, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Mitsuo Sekine

    1. Tokyo Institute of Technology, Yokohama, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • Presents the latest knowledge on a range of topics relating to the synthesis of therapeutic oligonucleotides
  • Aims to provide novel insights and inspire fresh ideas in nucleic acid chemistry
  • Meets the needs of both established researchers and students new to the field

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Table of contents (16 chapters)

  1. Front Matter

    Pages i-viii
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  2. Synthesis of Natural Oligonucleotides

    1. Front Matter

      Pages 1-1
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    2. Non-protected Synthesis of Oligonucleotides

      • Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine
      Pages 3-16

    3. Various Coupling Agents in the Phosphoramidite Method for Oligonucleotide Synthesis

      • Masaki Tsukamoto, Yoshihiro Hayakawa
      Pages 17-39

    4. Recent Development of Chemical Synthesis of RNA

      • Mitsuo Sekine
      Pages 41-65

    5. RNA Synthesis Using the CEM Group

      • Hidetoshi Kitagawa
      Pages 67-81

    6. Liquid-Phase Synthesis of Oligonucleotides

      • Satoshi Katayama, Kunihiro Hirai
      Pages 83-95

    7. Large-Scale Oligonucleotide Manufacturing

      • Eduardo Paredes, Tatsuya Konishi
      Pages 97-112

  3. Synthesis and Properties of Artificial Oligonucleotides

    1. Front Matter

      Pages 113-113
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    2. Nucleosides and Oligonucleotides Incorporating 2-Thiothymine or 2-Thiouracil Derivatives as Modified Nucleobases

      • Kohji Seio, Mitsuo Sekine
      Pages 115-130

    3. Site-Specific Modification of Nucleobases in Oligonucleotides

      • Yoshiyuki Hari
      Pages 131-145

    4. Four-Hydrogen-Bonding Base Pairs in Oligonucleotides: Design, Synthesis, and Properties

      • Noriko Saito-Tarashima, Akira Matsuda, Noriaki Minakawa
      Pages 147-169

    5. Photo-Cross-Linkable Artificial Nucleic Acid: Synthesis and Properties of 3-Cyanovinylcarbazole-Modified Nucleic Acids and Its Photo-Induced Gene-Silencing Activity in Cells

      • Takashi Sakamoto, Kenzo Fujimoto
      Pages 171-186

    6. Effects of 2′-O-Modifications on RNA Duplex Stability

      • Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine
      Pages 187-199

    7. 2′,4′-Bridged Nucleic Acids Containing Plural Heteroatoms in the Bridge Moiety

      • Yoshiyuki Hari, Satoshi Obika
      Pages 201-221

    8. Synthesis and Therapeutic Applications of Oligonucleotides Containing 2′-O,4′-C-Ethylene- and 3′-O,4′-C-Propylene-Bridged Nucleotides

      • Koji Morita, Makoto Koizumi
      Pages 223-232

    9. RNA Bioisosteres: Chemistry and Properties of 4′-thioRNA and 4′-selenoRNA

      • Noriaki Minakawa, Noriko Saito-Tarashima, Akira Matsuda
      Pages 233-252

    10. Development of Triplex Forming Oligonucleotide Including Artificial Nucleoside Analogues for the Antigene Strategy

      • Yosuke Taniguchi, Shigeki Sasaki
      Pages 253-269

    11. Chemical Synthesis of Boranophosphate Deoxy-ribonucleotides

      • Yohei Nukaga, Takeshi Wada
      Pages 271-284
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Keywords

About this book

This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.




Editors and Affiliations

  • Osaka University, Suita, Japan

    Satoshi Obika

  • Tokyo Institute of Technology, Yokohama, Japan

    Mitsuo Sekine

About the editors

Satoshi Obika, Osaka University, Japan

Mitsuo Sekine, Tokyo Institute of Technology, Japan

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