Yersinia Pestis Protocols

1. Front Matter

   Pages i-vi

   PDF

2. Isolation and Identification of Yersinia pestis

   • Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang et al.

   Pages 1-14

3. Isolation and Characterization of Yersinia pestis Bacteriophages

   • Xiangna Zhao

   Pages 15-22

4. Extraction of Chromosomal DNA and Plasmid from Yersinia pestis

   • Yazhou Zhou

   Pages 23-27

5. Extraction of Total RNA from Yersinia pestis

   • Haili Wang, Yanping Han

   Pages 29-34

6. Genotyping of Yersinia pestis

   • Yanjun Li, Yujun Cui

   Pages 35-59

7. Genome-Wide Variation Analysis of Yersinia pestis

   • Chao Yang, Yujun Cui

   Pages 61-66

8. Genome-Wide Detection of Expressed sRNAs in Yersinia pestis with RNA-seq

   • Yanping Han

   Pages 67-80

9. Validation of Yersinia pestis sRNAs with Nonradioactive-Labeled Northern Blotting

   • Yanping Han

   Pages 81-88

10. Determination of Transcription Start Sites (TSSs) in Yersinia pestis with a Primer Extension Assay

    • Yanping Han

    Pages 89-98

11. Purification of Hfq-Associated RNAs with RNA Immunoprecipitation (RIP)

    • Yanping Han

    Pages 99-106

12. Overexpression of Target sRNAs in Tightly Controlled Multicopy Plasmids

    • Xiaofang Gao, Zizhong Liu, Yanping Han

    Pages 107-113

13. Target Validation of sRNA with a GFP Reporter Gene Fusion System

    • Xiaofang Gao, Zizhong Liu, Yanping Han

    Pages 115-120

14. Inducible Gene Expression in Yersinia pestis

    • Juping Zhao, Yicheng Sun

    Pages 121-126

15. Analysis of Yops Secretion by Yersinia pestis Type III Secretion System

    • Zongmin Du, Shiyang Cao

    Pages 127-132

16. Co-immunoprecipitation Analysis for the Detection of Protein–Protein Interactions in Yersinia pestis

    • Shiyang Cao, Zongmin Du

    Pages 133-138

17. Detection of Type III Secretion System Needle Assembly in Yersinia pestis by Cross-Linking YscF

    • Shiyang Cao, Zongmin Du

    Pages 139-143

18. Evaluation of Protein Translocation into Eukaryotic Cells by Yersinia pestis

    • Yafang Tan

    Pages 145-150

19. Adhesion Analysis of Yersinia pestis to Host Cells

    • Yafang Tan

    Pages 151-156

20. Construction of Gene Deletion Mutants in Yersinia pestis

    • Wenliang Li, Dan Rong, Yanping Han

    Pages 157-163

This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.

• Y. pestis
• Genotyping
• Identification
• Genomic analysis
• Transcriptomic analysis
• Animal models

• Beijing Institute of Microbiology and Epidemiology, Beijing, China

  Ruifu Yang

Dr. Ruifu Yang is a professor at the Beijing Institute of Microbiology and Epidemiology and the Chief-Scientist for National Basic Research Program (973 Program). He serves as the vice president for the Chinese Society for Microbiology. His research focuses on bacterial pathogenesis using genomics- and proteomics-based techniques. He has published more than 100 articles on international well-known journals, such as Nature Genetics, PNAS, BMC Microbiology.  

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