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Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci

  • Book
  • © 1998

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Editors:
  1. Paula M. Fives-Taylor

    1. Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, USA

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  2. Donald J. LeBlanc

    1. Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, USA
      Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, USA

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Table of contents (27 chapters)

  1. Front Matter

    Pages i-xvi
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  2. Tn917 transposon mutagenesis and marker rescue of interrupted genes of Streptococcus mutans

    • Dennis G. Cvitkovitch, Juan A. Gutierrez, Paula J. Crowley, Laura Wojciechowski, Jeffrey D. Hillman, Arnold S. Bleiweis
    Pages 1-12

  3. Site-specific homologous recombination mutagenesis in group B streptococci

    • Harry H. Yim, Craig E. Rubens
    Pages 13-20

  4. Targeted mutagenesis of enterococcal genes

    • Xiang Qin, Fang Teng, Yi Xu, Kavindra V. Singh, George M. Weinstock, Barbara E. Murray
    Pages 21-33

  5. A lactococcal pWV01-based integration toolbox for bacteria

    • Kees Leenhouts, Gerard Venema, Jan Kok
    Pages 35-50

  6. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion

    • W. Michael McShan, Robert E. McLaughlin, Annika Nordstrand, Joseph J. Ferretti
    Pages 51-57

  7. Streptococcal integration vectors for gene inactivation and cloning

    • Lin Tao
    Pages 59-64

  8. Induction of transformation in streptococci by synthetic competence stimulating peptides

    • Peter Gaustad, Donald A. Morrison
    Pages 65-70

  9. Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

    • David A. Mills, Trevor G. Phister, Kathleen A. Baldwin, Gary M. Dunny, Larry L. McKay
    Pages 71-78

  10. Use of electroporation in genetic analysis of enterococcal virulence

    • Helmut Hirt, Yi Chen, Patrick M. Schlievert, Gary M. Dunny
    Pages 79-84

  11. Genetic transfer methods for Streptococcus sobrinus and other oral streptococci

    • Donald J. LeBlanc, Yi-Ywan Chen, Nicole D. Buckley, Linda N. Lee
    Pages 85-93

  12. Isolation of enterococcal antigen-encoding genes from genomic libraries

    • Yi Xu, Lingxia Jiang, Xiaomei Jin, Barbara E. Murray, George M. Weinstocks
    Pages 95-106

  13. A simple microtiter plate screening assay for bacterial invasion or adherence

    • Victor Nizet, Arnold L. Smith, Paul M. Sullam, Craig E. Rubens
    Pages 107-111

  14. End-probing: A non-radioactive approach to mapping transposon insertions

    • Martin H. Lee, Aphakorn Nittayajarn, Craig E. Rubens
    Pages 113-118

  15. A method for mapping phage-inducible promoters for use in bacteriophage-triggered defense systems

    • G. M. Djordjevic, T. R. Klaenhammer
    Pages 119-126

  16. Secretion of heterologous proteins by genetically engineered Streptococcus gordonii

    • Teruaki Shiroza, Howard Kuramitsu
    Pages 127-136

  17. Examination of streptococcal gene expression in the mammalian environment

    • W. Todd Grey, Joshua D. Lasker, Roy Curtiss III, Michael C. Hudson
    Pages 137-142

  18. Analysis of adherence-associated gene expression in Streptococcus parasangusis: A method for RNA isolation

    • Eunice H. Froeliger, Paula Fives-Taylor
    Pages 143-151

  19. Development of an integrative, lacZ transcriptional-fusion plasmid vector for Streptococcus mutans and its use to isolate expressed genes

    • Francesca Peruzzi, Patrick J. Piggot, Lolita Daneo-Moore
    Pages 153-163

  20. Use of proteomics and PCR to elucidate changes in protein expression in oral streptococci

    • Robert G. Quivey Jr, Wendi L. Kuhnert, Roberta C. Faustoferri
    Pages 165-179
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Keywords

About this book

This book is devoted entirely to methods developed in and for studies of members of the bacterial family Streptococcaceae. Many of the studies that have been conducted on the Streptococcaceae were initiated because of the diseases they cause, or to enhance their utility from an industrial perspective. However, the results of many of these investigations have demonstrated a complexity among some members of the family that warrants an interest in them in their own right, apart from or in addition to any biomedical or industrial considerations.
It is therefore hoped and expected that the advanced methods contained in this book will be of interest to those who work with the streptococci and other Gram-positive organisms, to researchers interested in industrial and medical microbiology and to any researcher who seeks to obtain a better understanding of how microorganisms interact with each other, their environment and their hosts.

Editors and Affiliations

  • Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, USA

    Paula M. Fives-Taylor

  • Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, USA

    Donald J. LeBlanc

  • Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, USA

    Donald J. LeBlanc

Bibliographic Information

  • Book Title: Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci

  • Editors: Paula M. Fives-Taylor, Donald J. LeBlanc

  • DOI: https://doi.org/10.1007/978-94-017-2258-2

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media Dordrecht 1998

  • Hardcover ISBN: 978-0-7923-5815-2Published: 31 July 1999

  • Softcover ISBN: 978-90-481-5262-9Published: 15 December 2010

  • eBook ISBN: 978-94-017-2258-2Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XVI, 256

  • Additional Information: Reprinted from METHODS IN CELL SCIENCE, 20:1-4....

  • Topics: Infectious Diseases, Human Genetics

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