Quantification of Tannins in Tree and Shrub Foliage

1. Front Matter

   Pages N3-x

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2. Chemical, Protein Precipitation and Bioassays for Tannins, Tannin Levels and Activity in Unconventional Feeds, and Efects and Fate of Tannins

   • Harinder P. S. Makkar

   Pages 1-42

3. Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual

   • Harinder P. S. Makkar

   Pages 43-48

4. Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method

   • Harinder P. S. Makkar

   Pages 49-51

5. Determination of Condensed Tannins (Proanthocyanidins)

   • Harinder P. S. Makkar

   Pages 53-54

6. Gallotannin Determination by Rhodanine Assay

   • Harinder P. S. Makkar

   Pages 55-56

7. Gallotannin Determination Using HPLC

   • Harinder P. S. Makkar

   Pages 57-58

8. Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate

   • Harinder P. S. Makkar

   Pages 59-61

9. Determination of Protein-Precipitable Phenolics

   • Harinder P. S. Makkar

   Pages 63-69

10. Protein-Binding Capacity by Filter Paper Assay

    • Harinder P. S. Makkar

    Pages 71-72

11. Radial Diffusion Assay

    • Harinder P. S. Makkar

    Pages 73-74

12. Tannin Bioassay

    • Harinder P. S. Makkar

    Pages 75-81

13. Radiolabelled BSA Precipitation Method

    • Harinder P. S. Makkar

    Pages 83-85

14. Simplified Radiolabelled BSA Precipitation Method

    • Harinder P. S. Makkar

    Pages 87-88

15. Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC)

    • Harinder P. S. Makkar

    Pages 89-93

16. References

    • Harinder P. S. Makkar

    Pages 95-96

17. Summary of Results from the First Phase of the Co-Ordinated Research Project

    • Harinder P. S. Makkar

    Pages 97-100

18. Back Matter

    Pages 101-102

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Here is the most complete guide available for the analysis of tannins. A battery of tannin methodologies is presented in a simple, clear and easy-to-understand manner. This unique guide covers chemical, biological and radio isotopic tannin assays. Comprehensive step-by-step protocols are presented for each method. The protocols enable non-specialists and specialists alike to implement the methods easily in the laboratory. It is an ideal laboratory manual for research scientists, graduate students, and laboratory personnel working in the fields of animal nutrition, soil nutrient management, wild life-plant interactions, and plant breeding.

• nutrition
• paper
• plant
• plant breeding
• protein
• research
• soil
• tree

• Animal Production & Health Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria

  Harinder P. S. Makkar

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