Selected Topics in Mass Spectrometry in the Biomolecular Sciences

1. Front Matter

   Pages i-xxvi

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2. Methods in Mass Spectrometry

   1. Front Matter

      Pages 1-1

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   2. Electrospray Ionization: Theory and Application

      • Simon J. Gaskell, Mark S. Bolgar, Isabel Riba, Scott G. Summerfield

      Pages 3-16

   3. Atmospheric Pressure Ionization (ISI and APCI)

      • A. Raffaelli

      Pages 17-31

   4. Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applications

      • M. Karas, U. Bahr

      Pages 33-53

   5. High Sensitivity ESI MS and MALDI MS

      • Terry B. Farmer, Richard M. Caprioli

      Pages 55-84

   6. Optimised GC/MS interfacing systems and operating conditions

      • P. J. Arpino

      Pages 85-101

   7. Quantitative Analysis by Mass Spectrometry: Some Important Considerations

      • M. W. Duncan

      Pages 103-119

   8. Optimization of Mass Spectrometric Ionisation Efficiency Data

      • İ. T. Özgen, O. Altungöz, B. Salih, B. G. M. Vandeginste

      Pages 121-127

   9. Role of Internal Energy in Mass Spectrometric Fragmentation

      • Károly Vékey

      Pages 129-142

   10. Interaction of Metal Ions and Protons with Simple Biomolecules. Structure Information from Bond Energies and Entropy Effects

       • B. A. Cerda, M. J. Nold, C. Wesdemiotis

       Pages 143-161

   11. A Promising Marriage Between Theory and Experiment: Density Functional Method Versus Mass Spectrometry

       • T. Marino, N. Russo, E. Sicilia, M. Toscano

       Pages 163-179

3. Instrumentation for Mass Analysis and Detection

   1. Front Matter

      Pages 181-181

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   2. Recent Advances in Magnet Sector Mass Spectrometry

      • O. V. Nemirovskiy, J. K. Gooden, R. Ramanathan, M. L. Gross

      Pages 183-211

   3. Quadrupole Mass Filters and Quadrupole Ion Traps

      • R. Graham Cooks, Guodong Chen, Carsten Weil

      Pages 213-238

   4. Time-of-Flight Mass Spectrometry

      • Marvin L. Vestal

      Pages 239-262

   5. Combined Analyzer Technologies

      • Peter Kofel, Urs P. Schlunegger

      Pages 263-286

   6. Fourier Transform Mass Spectrometry

      • Michael L. Easterling, I. Jonathan Amster

      Pages 287-314

4. Application to Biomolecules

   1. Front Matter

      Pages 315-315

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   2. Protein Characterization by MS in the Pharmaceutical Industry

      • D. Müller, P. Schindler, M. Coulot, W. J. Richter, W. Maerki, H. Grossenbacher

      Pages 317-345

Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases.
Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section.
Audience: Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph.D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories.

• DNA
• Lipid
• Mass Spectrometry
• Nucleotide
• Polysaccharid
• Termination
• proteins

• Medical School, University of Texas, Houston, USA

  Richard M. Caprioli

• SESMA-C.N.R., Napoli, Italy

  Antonio Malorni

• Dipartimento di Chimica, Arcavacata di Rende, Università della Calabria, Italy

  Giovanni Sindona

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