Cell Culture Engineering VI

1. Front Matter

   Pages i-vi

   PDF

2. Cell therapy in kidney failure

   • H. David Humes, Angela J. Funke, Deborah A. Buffington

   Pages 1-8

3. Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)

   • Teri L. Aldrich, James N. Thomas, Arvia E. Morris

   Pages 9-17

4. Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination

   • Andrea Hawerkamp, Dirk Lütkemeyer, Frank Gudermann, Anna Falkenhain, Heino Büntemeyer, Jürgen Lehmann

   Pages 19-29

5. Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line

   • Brian Rasmussen, Ray Davis, James Thomas, Pranhitha Reddy

   Pages 31-42

6. Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them

   • Stephen J. Wessman, Randall L. Levings

   Pages 43-48

7. An overview of viral and viral-like agents in cell culture systems

   • John C. Petricciani

   Pages 49-52

8. New adenovirus vectors for protein production and gene transfer

   • Bernard Massie, Dick D. Mosser, Maria Koutroumanis, Isabelle Vitté-Mony, Linda Lamoureux, France Couture et al.

   Pages 53-64

9. Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue

   • František Franěk, Antonín Holý, Ivan Votruba, Tomáš Eckschlager

   Pages 65-72

10. Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth — associated production of a foreign protein, β-galactosidase

    • Frank W. F. Lee, Cynthia B. Elias, Paul Todd, Dhinakar S. Kompala

    Pages 73-80

11. A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy

    • G. Schoofs, T. J. Monica, J. Ayala, J. Horwitz, T. Montgomery, G. Roth et al.

    Pages 81-89

12. Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3

    • Noelle-Anne Sunstrom, Masood Baig, Louise Cheng, Derick Payet Sugyiono, Peter Gray

    Pages 91-99

13. Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

    • James Varani, Felicia Piel, Sean Josephs, Ted F. Beals, William J. Hillegas

    Pages 101-109

14. Regulated multicistronic expression technology for mammalian metabolic engineering

    • Martin Fussenegger, Samuel Moser, James E. Bailey

    Pages 111-125

15. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions

    • Antonio De León, Héctor Mayani, Octavio T. Ramírez

    Pages 127-138

16. Historical reflections on cell culture engineering

    • Anthony S. Lubiniecki

    Pages 139-145

17. Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation

    • Janet H. Parham, Marie A. Iannone, Laurie K. Overton, Jeff T. Hutchins

    Pages 147-155

18. Population balance model of in vivo neutrophil formation following bone marrow rescue therapy

    • L. K. Nielsen, J. G. Bender, W. M. Miller, E. T. Papoutsakis

    Pages 157-162

19. Mammalian cell retention devices for stirred perfusion bioreactors

    • Steven M. Woodside, Bruce D. Bowen, James M. Piret

    Pages 163-175

20. Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells

    • A. Perani, R. P. Singh, R. Chauhan, M. Al-Rubeai

    Pages 177-188

The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.

• Expression
• Mammalia
• Nucleotide
• Tissue Engineering
• Viruses
• Vivo
• biotechnology
• gene expression
• gene therapy
• gene transfer
• metabolism

• The Johns Hopkins University, Baltimore, USA

  Michael J. Betenbaugh, Alison J. Mastrangelo

• The Ohio State University, Columbus, USA

  Jeffrey J. Chalmers

• Genentech Inc., South San Francisco, USA

  Rob Arathoon

• Oregon State University, Corvallis, USA

  Frank W. R. Chaplen

Policies and ethics