Plant Genomes: Methods for Genetic and Physical Mapping

1. Front Matter

   Pages i-xi

   PDF

2. Simple plant DNA isolation procedures

   • Kimberlee K. Kidwell, Thomas C. Osborn

   Pages 1-13

3. Methods of Southern blotting and hybridization

   • Robert Bernatzky, Angela Schilling

   Pages 15-33

4. Organelle DNA isolation and RFLP analysis

   • Jeffrey D. Palmer

   Pages 35-53

5. Detection of DNA sequence variation for genome analysis

   • Donna M. Shattuck-Eidens, Russell N. Bell, Timothy Helentjaris

   Pages 55-70

6. Pulsed-field gel electrophoresis

   • Raymond Van Daelen, Pim Zabel

   Pages 71-100

7. Physical mapping by random clone fingerprint analysis

   • Brian M. Hauge, Howard M. Goodman

   Pages 101-139

8. Construction and application of a YAC library

   • Hans M. Albertsen, Hadi Abderrahim, Daniel Cohen, Denis Le Paslier

   Pages 141-165

9. Linkage analysis in human genetics

   • Jean-Marc Lalouel

   Pages 167-180

10. Statistical methodologies for mapping and analysis of quantitative trait loci

    • Joel Ira Weller

    Pages 181-207

11. Mapping quantitative trait loci using nonsimultaneous and simultaneous estimators and hypothesis tests

    • S. J. Knapp, W. C. Bridges, B.-H. Liu

    Pages 209-237

12. Epilogue: additional reflections

    • Jacques S. Beckmann

    Pages 239-245

13. Back Matter

    Pages 247-250

    PDF

JACQUES S. BECKMANN & THOMAS C. OSBORN Extraordinary progress has been made in the analyses of the genetic structures of higher eukaryotic genomes. Only ten years elapsed between the initial proposals to use molecular DNA markers for the generation of a complete linkage map of the human genome [5, 17] and the first description of a 10 centimorgan map of one of its chromosomes [22], soon to be followed by others. The availability of molecular DNA markers, henceforth called genomic markers [for a review of their properties see 1, 2, 20], represents a milestone in genetics by providing the capacity for complete genetic coverage of all genomes. It is important to remember that the nature of the DNA polymorphism or of the specific method used to uncover it can be quite different for different marker loci. The genetic variation detected can be a result of a simple point mutation, a DNA insertion/deletion event, or a change in repeat copy number at some hypervariable DNA [11] or micro satellite [21] motif. Currently, the methods of detection can involve use of restriction endonucleases, nucleic acid hybridization, or DNA sequence amplification. Each of these sources of var­ iation and methods of detection can have utility for different applications. Furthermore, new approaches for the detection of DNA polymorphism are constantly emerging. The primary concern here is that the monitored poly­ morphism defines a genetic marker 'useful' for the desired application.

• DNA
• Organe
• Organelle
• YAC
• hybridization

• Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel

  J. S. Beckmann

• Department of Agronomy, University of Wisconsin, Madison, USA

  T. C. Osborn

Policies and ethics