Chiral Liquid Chromatography

1. Front Matter

   Pages i-ix

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. Molecular asymmetry

      • S. A. Matlin

      Pages 3-13

   3. The importance of enantiomer separations

      • W. H. De Camp

      Pages 14-22

   4. Chiral liquid chromatography: past and present

      • A. Pryde

      Pages 23-35

3. Chiral derivatization

   1. Front Matter

      Pages 37-37

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   2. Chiral derivatization

      • M. Ahnoff, S. Einarsson

      Pages 39-80

4. Direct chiral resolution

   1. Front Matter

      Pages 81-81

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   2. Chiral ligand exchange chromatography

      • S. Lam

      Pages 83-101

   3. Synthetic multiple-interaction chiral bonded phases

      • T. D. Doyle

      Pages 102-128

   4. Immobilized proteins as HPLC chiral stationary phases

      • I. W. Wainer

      Pages 129-147

   5. Cyclodextrin inclusion complexation

      • L. Coventry

      Pages 148-165

   6. Binding to cellulose derivatives

      • D. M. Johns

      Pages 166-176

   7. Binding to synthetic polymers

      • D. M. Johns

      Pages 177-184

   8. Ion-pairing

      • G. Szepesi

      Pages 185-202

   9. Other direct chiral resolution methods

      • W. J. Lough

      Pages 203-209

5. Strategy for development of LC enantiomeric determination methods

   1. Front Matter

      Pages 211-211

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   2. Consideration of other techniques

      • W. J. Lough, S. A. Matlin

      Pages 213-222

   3. Choice of chiral LC systems

      • W. J. Lough, I. W. Wainer

      Pages 223-234

   4. Optimization

      • T. A. G. Noctor, A. F. Fell, B. Kaye

      Pages 235-243

While working as a chromatographer in the pharmaceutical industry, it became apparent to the editor that there was a pressing need for a comprehensive reference text for analysts working on the resolution of enantiomers by liquid chromatography (LC). This need arises from the fact that, whereas previously it was very difficult to determine enantiomers by direct means, there is now a wide choice of direct LC methods. At the same time, regulatory authorities have been changing their attitudes towards the administration of pharmaceuticals as racemates, partly because it is now possible to study the individual enantiomers. Clearly this abundance of new information needs to be rationalized. More importantly, the chiral LC systems which are commercially available or readily accessible to the practising chromatographer needed to be reviewed and, to a much greater extent than in existing reviews or books, discussed in terms of their practical application. Accordingly this book is very much orientated towards the practical aspects of these commercially available and readily accessible chiral LC systems. To this end, it is written for practising chromatographers by a team of practising, experienced chromatographers who have spent many years tackling the problems presented by resolving enantiomers by LC. The practical aspects of common chiral LC systems cannot be fully understood if discussed in isolation.

• Enantiomer
• chromatography
• information
• optimization
• pharmaceutical
• pharmaceutical industry
• pharmaceuticals
• racemate

• School of Pharmaceutical and Chemical Sciences, University of Sunderland, Sunderland, UK

  W. J. Lough

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