Safety in Cell and Tissue Culture

1. Front Matter

   Pages i-ix

   PDF

2. Source Materials

   • Glyn Stacey, Alan Doyle, David Tyrrell

   Pages 1-25

3. Cell biology aspects of safety in cell culture

   • Trevor Littlewood

   Pages 26-48

4. Viral contamination of cell cultures

   • Alan Jennings

   Pages 49-63

5. Laboratory practice

   • Bruce Jones

   Pages 64-86

6. Planning and design of a cell and tissue culture laboratory

   • Christopher B. Morris

   Pages 87-101

7. Quality control and validation

   • Alan Doyle, Glyn Stacey

   Pages 102-115

8. Containment facilities: design, construction and working practices

   • John Benbough, B. Andrew Curran

   Pages 116-130

9. Scale-up of animal cell culture systems

   • Bryan Griffiths, Wolfgang Noe

   Pages 135-154

10. Production and containment of bioreactor processes

    • Geoffrey Leaver

    Pages 155-172

11. Risk assessment

    • Heather Sheeley

    Pages 173-188

12. Safety aspects of genetic modification procedures

    • Caroline MacDonald

    Pages 189-204

13. International guidelines for safe packaging and transport of biological materials

    • Christine Rohde, Dieter Claus

    Pages 205-222

14. Back Matter

    Pages 223-244

    PDF

It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con­ tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven­ titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon­ sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.

• biological materials
• biology
• cell biology
• classification
• microorganism
• tissue

`This book is interesting first at all for biotechnologists and for industrial companies which all should urgently buy this book.'
Cellular and Molecular Biology, 45:4 (1999)

• National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK

  G. Stacey

• Centre for Applied Microbiology and Research, European Collection of Cell Cultures, Salisbury, Wiltshire, UK

  A. Doyle

• Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK

  P. Hambleton

Policies and ethics