Animal Cell Culture and Production of Biologicals

1. Front Matter

   Pages i-xi

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2. Control of Animal Cell Growth

   1. Novel Specific Inhibitors for Analysis of Eukaryotic Cell Cycle Control

      • Teruhiko Beppu, Minoru Yoshida

      Pages 1-10

3. New Cell Lines

   1. Transgenic Animals as a Source of Genetically-Engineered Trans-Immortalised Cell Lines

      • S. Jallat, F. Perraud, W. Dalemans, A. Balland, T. Faure, P. Meulien et al.

      Pages 11-19

   2. CD8+ Suppressor T Cell Clone 13G2 Secretes a Suppressive Lymphokine, Immune Suppressive Factor-T (ISF-T)

      • Tatsuhiro Hisatsune, Ken-Ichi Nishijima, Yuji Minai, Atsushi Enomoto, Shuichi Kaminogawa

      Pages 21-26

   3. Development, Availability and Characterization of ATCC Human and Animal Cell Lines

      • R. J. Hay

      Pages 27-39

4. Cell Structure System Including Serum-Free Culture

   1. Recent Advances in Animal Cell Biotechnology

      • R. E. Spier

      Pages 41-46

   2. Critical Analysis of Process Development on in-Vitro Growth of Chick-Embryo

      • A. Venkataraman, J. Anjani Kumari, P. S. R. Babu, T. Panda

      Pages 47-52

   3. Improved Method for Inoculation of a Cell Suspension Into a Hollow Fiber Bioreactor

      • George Avgerinos, Sean Downing, Beth Gefre, Harvey Freedman, Christina Pelletier

      Pages 53-60

   4. Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion

      • H. D. Blasey, V. Jäger

      Pages 61-73

   5. Cultivation of Hepatocytes in a New Entrapment Reactor: a Potential Bioartificial Liver

      • Wei-Shou Hu, Scott L. Nyberg, Russell A. Shatford, William D. Payne, Frank B. Cerra

      Pages 75-80

   6. Continuous Culture with Cell Precipitation for Recombinant Protein Production

      • H. R. Kim, B. H. Chung, C. H. Kim, I. S. Chung

      Pages 81-85

   7. Elimination of Microorganisms from Cell Culture Medium Using Regenerated Cellulose Hollow Fiber (BMM)

      • S. Manabe, M. Umeda, A. Kono, I. Togo, S. Fukada, K. Yamaguchi

      Pages 87-94

   8. Effects of Shear Stress on the Growth of Hybridoma Cells Cultivated in Serum-Free Medium Coupled with Ammonia Removing System

      • F. R. Nayve Jr., M. Matsumura, H. Kataoka

      Pages 95-101

   9. GMP Production of Biopharmaceuticals Using High Density, Fluidized-Bed Cell Culture Technology

      • Peter W. Runstadler, Michael W. Young

      Pages 103-119

   10. Anchorage-Dependent Animal Cell Growth in Porous Microcarrier Culture

       • N. Shiragami, Y. Ohira, H. Unno

       Pages 121-126

   11. Formation of Multicellular Aggregates of Adult Rat Hepatocytes

       • Yasuyuki Sakai, Motoyuki Suzuki

       Pages 127-134

   12. Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells

       • B. Bugarski, G. Vunjak, G. Jovanovic, K. Cuperlovic, M. F. Goosen

       Pages 135-140

   13. Simulation of Growth of Hybridoma Cells Immobilized in Alginate Gel Beads Based on an Oxygen Limited Model

       • Y. Shirai, K. Hashimoto, A. Kubo

       Pages 141-149

   14. High Density Suspension Culture of Insect Cells in a Stirred Bioreactor

       • S. Deutschmann, V. Jäger

       Pages 151-158

   15. Media for Cultivation of Animal Cells: An Overview

       • A. Mizrahi, A. Lazar

       Pages 159-180

In the past two decades, the importance of animal cell technology has increased enormously. First, useful proteins can be produced by cultured animal cells, in which the desired product can be modified and organized so as to retain its biological function. Second, studies of cultured cells can provide information needed to understand molecular mechanisms that govern what happens in tissues, organs, and even entire organisms. For this second purpose, biochemists and molecular biologists may need a large number of such cells. Third, cultured cells can be used instead of tissues and organs clinically. The Third Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), at which participants from abroad were warmly welcomed, was held in Kyoto on December 11-13, 1990. It was organized around the idea of providing a place for the review of much new data on such applications of cultured cells and for exchanges of the views of the participants about progress in the field. This volume, divided into seven sections, contains the proceedings of the meeting. The first section reviews the molecular basis of the control of animal cell growth. In the following sections, physicochemical and biochemical factors for cell growth and production of biologicals, cell culture systems including serum-free culture, new cell lines, specific products and their characteristics, and in vitro assays for toxic, carcinogenic, and pharmacological effects are taken up in their tum.

• cell
• cell culture

• Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto, Japan

  Ryuzo Sasaki, Koji Ikura

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