In Situ Hybridization: Medical Applications

1. Front Matter

   Pages i-xii

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2. Techniques for in situ hybridization histochemistry

   • John McCafferty, Carl Alldus

   Pages 1-36

3. Use of comparative in situ hybridization and immunocytochemistry for the study of regulatory peptides

   • Giorgio Terenghi, Julia M. Polak

   Pages 37-51

4. Application of in situ hybridization to studies of messenger RNA in the nervous system: functional correlations and their quantitation

   • J. de Belleroche, L. Virgo, A. Rashid, Y. Collaço Moraes

   Pages 52-65

5. The use of in situ hybridization in studies of viral disease

   • Adrienne L. Morey, Kenneth A. Fleming

   Pages 66-96

6. In situ hybridization for molecular cytogenetics

   • A. K. Raap, C. J. Cornelisse

   Pages 97-109

7. Back Matter

   Pages 111-116

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In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. The great strength of this approach is the ability to relate the distribution of specific nucleic acids with cell structures and the protein products of the target gene by means of immunohistochemistry. Complementary DNA, RNA or oligonucleotide probes, suitably labelled, are hybridized to specific DNA or RNA targets within tissues. The spatial information thus obtained has contributed greatly to our understanding of such diverse areas of research as gene mapping, viral infection, cytogenetics, protein synthesis, prenatal diagnosis and tissue grafting.
This book is not intended as another recipe book, although it does describe theoretical and practical aspects of the technology. Rather, the authors critically describe the contribution made by in situ hybridization to specific areas of medical research.

• Chromosom
• DNA
• genetics
• hybridization
• tissue

• Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK

  G. R. Coulton, J. Belleroche

Policies and ethics