Mass Spectrometry in the Biological Sciences: A Tutorial

1. Front Matter

   Pages i-xxi

   PDF

2. Instrumentation for Mass Analysis and Detection

   1. Front Matter

      Pages 1-1

      PDF

   2. Sectors and Tandem Sectors

      • J. R. Trainor, P. J. Derrick

      Pages 3-27

   3. Hybrid Tandem Mass Spectrometry

      • Simon J. Gaskell, Kevin D. Ballard

      Pages 29-58

   4. Triple Quadrupole Mass Spectrometry

      • V. H. Wysocki

      Pages 59-77

   5. Quadrupole Ion Trap Mass Spectrometers for Studies of Biomolecules

      • R. Cameron Dorey

      Pages 79-92

   6. Fourier Transform Mass Spectrometry: Features, Principles, Capabilities, and Limitations

      • Cliffton B. Jacoby, Christopher L. Holliman, Michael L. Gross

      Pages 93-116

   7. Time-of-Flight Mass Spectrometers

      • H. Wollnik, U. Grüner, G. Li

      Pages 117-131

   8. Ion Detection in Mass Spectrometry

      • Paul W. Geno

      Pages 133-142

3. Methods in Mass Spectrometry

   1. Front Matter

      Pages 143-143

      PDF

   2. Electrospray Mass Spectrometry

      • M. Mann, S. Shen, J. B. Fenn

      Pages 145-163

   3. Laser Desorption Mass Spectrometry. Part I: Basic Mechanisms and Techniques

      • F. Hillenkamp, H. Ehring

      Pages 165-179

   4. Laser Desorption Mass Spectrometry. Part II Performance and Applications of Matrix-Assisted Laser Desorption/Ionization of Large Biomolecules

      • A. Overberg, A. Hassenbürger, F. Hillenkamp

      Pages 181-197

   5. Laser Desorption, Chemical Ionization, and Laser Desorption/Chemical Ionization Applications with Fourier Transform Mass Spectrometry

      • J. Paul Speir, Greg S. Gorman, I. Jonathan Amster

      Pages 199-212

   6. Plasma Desorption Mass Spectrometry: Principles and Applications to Protein Studies

      • Peter Roepstorff

      Pages 213-227

   7. Continuous-Flow Fast Atom Bombardment Mass Spectrometry in Analytical Biochemistry

      • William T. Moore, Richard M. Caprioli

      Pages 229-252

   8. Combined Liquid Chromatography/Mass Spectrometry (LC/MS): A Review

      • P. J . Arpino

      Pages 253-267

   9. Combined Supercritical Fluid Chromatography/Mass Spectrometry (SFC/MS)

      • P. J. Arpino

      Pages 269-280

   10. Determination of Biomolecules by Using Liquid Chromatography and Thermospray Mass Spectrometry: From Penicillins to Peptides

       • Somchai Suwanrumpha, Matthew A. Mclean, Saul W. Fink, Cheryl Wilder, Kristina Stachowiak, Douglas F. Dyckes et al.

       Pages 281-301

   11. Neutralization-Reionization Mass Spectrometry: Fundamental Studies and Analytical Applications

       • M. J. Polce, C. Wesdemiotis

       Pages 303-310

The developments in mass spectrometry over the past fifteen years have been impressive in their implications in bioanalytical chemistry. The achievements begin with the inventions of Cf-252 Plasma Desorption Mass Spectrometry by Macfarlane and Fourier Transform Mass Spectrometry by Comisarow and Marshall in the mid 1970s. The former showed the feasibility of producing large gas-phase ions from large biomolecules whereas the latter enhanced the capabilities for ion trapping especially in analytical mass spectrometry. A major achievement was the development by Barber of Fast Atom Bombardment (FAB) mass spectrometry, an advance that heralded a new era in biological mass spectrometry. Contemporary and routine instruments such as magnetic sectors and quadrupoles were rapidly adapted to F AB, and nearly the entire universe of small molecules became amenable to study by mass spectrometry. The introduction of FAB also paved the way for improvement of instrument capability. For example, the upper mass limit of magnet sector mass spectrometers was increased by nearly an order of magnitude by the instrument manufacturers. Furthermore, the technique of tandem mass spectrometry (MS/MS) was given new meaning because important structural information for biomolecules could now be produced for ions introduced by FAB into the tandem instrument. The evolution of MS/MS continues today with the development of ion traps, time-of-flight, and sector instruments equipped with array detection.

• Mass Spectrometry
• Nucleotide
• bacteria
• biochemistry
• cell membrane
• chemistry
• protein
• proteins
• supercritical fluid
• translation

`I therefore can be highly recommended to all chemists and physicist who are interested to learn about the current applicability of mass spectrometry to biological sciences and in particular to those who are practising this area of research.'
Recueil dex Travaux Chimiques des Pays-Bas
'In conclusion, Mass Spectrometry in the sciences: A Tutorial achieves its objective as a tutorial for biological mass spectrometry. This book would be an excellent complement to an introductory mass spectrometry book because it contains the most recent techniques...covered by most introductory texts....I recommend this book as an ideal primer to understanding the current status of biological mass spectrometry' 'Applied Spectroscopy' 46:10 1992

• Midwest Center for Mass Spectrometry, Department of Chemistry, University of Nebraska-Lincoln, Lincoln, USA

  Michael L. Gross

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