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Gas Enzymology

Proceedings of a Symposium held at Odense University, Denmark, 28–29 May 1984

  • Conference proceedings
  • © 1985

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Editors:
  1. H. Degn

    1. Institutes of Biochemistry and Chemistry, Odense University, Odense, Denmark

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  2. R. P. Cox

    1. Institutes of Biochemistry and Chemistry, Odense University, Odense, Denmark

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  3. H. Toftlund

    1. Institutes of Biochemistry and Chemistry, Odense University, Odense, Denmark

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Table of contents (16 papers)

  1. Front Matter

    Pages i-x
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  2. Quadrupole Mass Spectrometric Measurement of Dissolved and Free Gases

    • Sándor Bohátka
    Pages 1-16

  3. Direct Mass-Spectrometric Monitoring of the Metabolism and Isotope Exchange in Enzymic and Microbiological Investigations

    • Y. M. Berlier, B. Dimon, G. Fauque, P. A. Lespinat
    Pages 17-35

  4. Simultaneous Dissolved Oxygen and Redox Measurements: Use of Polarographic, Bioluminescence and Mass Spectrometric Monitoring Combined with Dual-Wavelength Spectrophotometry

    • D. Lloyd
    Pages 37-53

  5. Alcohol Oxidase in Candida Boidinii, Combined Spectrophotometry and Oxygen Measurements during Ethanol Inactivation

    • D. Hill, D. Lloyd
    Pages 55-60

  6. Mechanism of Activation and Reduction of Dioxygen by Rhus Laccase — A Blue Copper Oxidase

    • Ole Farver
    Pages 61-78

  7. Dioxygen Reduction in Blue Oxidases: The Electron Transfer and Protonation Steps

    • Bengt Reinhammar
    Pages 79-89

  8. Photosynthesis Studies Using Mass Spectrometric Techniques

    • Richard Radmer, Otto Ollinger
    Pages 91-104

  9. Interactions of Carbon Dioxide and Oxygen on D-Ribulose 1,5-Bisphosphate Carboxylation

    • A. J. Keys, N. P. Hall, M. A. J. Parry, C. N. G. Schmidt, S. Gutteridge
    Pages 105-120

  10. The Catalytic Mechanism of Carbonic Anhydrase

    • Sven Lindskog
    Pages 121-133

  11. The Catalytic Mechanism of Carbonic Anhydrase Studied by 18O Exchange

    • D. N. Silverman, C. K. Tu, G. C. Wynns
    Pages 135-145

  12. Enzymes Oxidizing Carbon Monoxide

    • Ortwin Meyer, Klaus Fiebig
    Pages 147-168

  13. Mechanistic Studies on the Mode of Action of Methane Monooxygenase

    • H. Dalton, D. J. Leak
    Pages 169-186

  14. The Reaction Mechanism of Methane Monooxygenase Studied by Membrane-Inlet Mass Spectrometry in Whole Cells of Methanotrophic Bacteria

    • Lars Joergensen
    Pages 187-200

  15. Continuous Monitoring of Fermentation Gases in an Artificial Rumen System (Rusitec) Using A Membrane-Inlet Probe on A Portable Quadrupole Mass Spectrometer

    • K. Hillman, D. Lloyd, A. G. Williams
    Pages 201-206

  16. Isotope Ratio Mass-Spectrometry Studies of HD Formation by Nitrogenase

    • Bent Borg Jensen
    Pages 207-227

  17. Binding and Activation of Dinitrogen in Nitrogenase

    • G. J. Leigh, J. R. Postgate
    Pages 229-246

  18. Back Matter

    Pages 247-250
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Keywords

About this book

Being small, shapeless and inert a gas molecule does not seem to be an enzyme's dream of a substrate. Nevertheless evolution has provided a host of enzymes which can interact specifically with gas molecules such as oxygen, carbon dioxide, nitrogen, hydrogen etc. Many of these enzymes play dominant roles on the world scene in biogeochemical cycles. On the cellular level they tend to be closely connected to the energy conserving apparatus. We define Gas Enzymology as the study of these enzymes. Historically, Gas Enzymology is a subspecialty of bioenergetics. Its foundations, technical as well as conceptual were laid by Warburg in his studies of the cellular combustion of nutrients. The Warburg apparatus supported the first thirty years of research in the field. It was succeeded by the Clark electrode which had its heyday during the period when the modern concepts of bioenergetics took shape. The Clark electrode, itself approaching thirty years of age, is now being sup­ plemented and in some cases replaced by the vastly more powerful membrane inlet mass spectrometer which measures with equal ease all dis­ solved gases of interest in biochemistry. It is our belief that future development of Gas Enzymology will be linked to the widespread exploit­ ation of this technique.

Editors and Affiliations

  • Institutes of Biochemistry and Chemistry, Odense University, Odense, Denmark

    H. Degn, R. P. Cox, H. Toftlund

Bibliographic Information

  • Book Title: Gas Enzymology

  • Book Subtitle: Proceedings of a Symposium held at Odense University, Denmark, 28–29 May 1984

  • Editors: H. Degn, R. P. Cox, H. Toftlund

  • DOI: https://doi.org/10.1007/978-94-009-5279-9

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: D. Reidel Publishing Company, Dordrecht, Holland 1985

  • Hardcover ISBN: 978-90-277-1900-3Due: 31 December 1984

  • Softcover ISBN: 978-94-010-8831-2Published: 09 February 2012

  • eBook ISBN: 978-94-009-5279-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 264

  • Topics: Enzymology, Physical Chemistry, Science, Humanities and Social Sciences, multidisciplinary

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