Application of Monoclonal Antibodies in Tumor Pathology

1. Front Matter

   Pages I-XII

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2. Basic and Technical Aspects

   1. Front Matter

      Pages 1-1

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   2. Selection of Monoclonal Antibodies.

      • S. O. Warnaar

      Pages 3-21

   3. Immunocytochemical Techniques.

      • F. T. Bosman

      Pages 23-36

   4. Double Immunoenzyme Staining Methods with Special Reference to Monoclonal Antibodies.

      • H. Mullink, D. M. Boorsma, S. C. Henzen-Logmans, C. J. L. M. Meijer

      Pages 37-47

3. Tumor Associated Antigens

   1. Front Matter

      Pages 49-49

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   2. Monoclonal Antibodies to Oncofetal Antigens.

      • M. Nap

      Pages 51-63

   3. Cytoskeletal Proteins as Markers in Surgical Pathology.

      • F. C. S. Ramaekers, A. Huijsmans, G. Schaart, O. Moesker, G. P. Vooijs

      Pages 65-85

   4. Immunological marker analysis of cells in the various hematopoietic differentiation stages and their malignant counterparts.

      • J. J. M. van Dongen, H. J. Adriaansen, H. Hooijkaas

      Pages 87-116

   5. Monoclonal Antibodies in Lymphoma Diagnosis and Classification.

      • S. Poppema, H. Hollema

      Pages 117-130

   6. Monoclonal Antibodies Recognizing Melanoma-Associated Antigens.

      • D. J. Ruiter, E-B. Broecker, C. Vennegoor, S. Ferrone

      Pages 131-165

   7. Monoclonal Antibodies in Breast Tumor Pathology.

      • Ph. C. Hageman, J. L. Peterse, J. Hilkens, J. Hilgers

      Pages 167-190

   8. Monoclonal Antibodies in Clinical and Experimental Pathology of Lung Cancer

      • L. De Leij, J. Broers, F. Ramaekers, H. Berendsen, Sj. Sc. Wagenaar

      Pages 191-210

   9. Monoclonal Antibodies in Diagnosis and Prognosis of Colorectal Cancer.

      • J. W. Arends, T. Wiggers, C. P. H. J. Verstijnen, F. T. Bosman

      Pages 211-222

   10. Monoclonal Antibodies Reacting with Tumors of the Urological Tract or Prostate

       • S. O. Warnaar, E. Oosterwijk

       Pages 223-235

   11. Ovarian Cancer

       • G. J. Fleuren

       Pages 237-254

   12. Endocrine Tumors.

       • A. C. Nieuwenhuijzen Kruseman

       Pages 255-263

   13. Application of Monoclonal Antibodies in Cytopathology.

       • G. P. Vooijs, A. C. L. M. Huysmans, M. H. J. Salet-V. D. Pol, A. J. M. Aspert-Van Erp, F. C. S. Ramaekers

       Pages 265-281

   14. Tumor Associated Antigens and Oncogene Products Defined by Monoclonal Antibodies

       • A. Thor, N. Ohuchi, P. Horan Hand, M. O. Weeks, W. W. Johnston, C. A. Szpak et al.

       Pages 283-296

4. Practical Application and Case Presentations

   1. Front Matter

      Pages 297-297

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The development of monoclonal antibodies to human tumor associated antigens has greatly facilitated the application of immunohistochemical techniques to analyze surgically removed tissues. During the last few years this approach has been utilized by a progressively increasing number of investigators to analyze malignant cells. Although monoclonal antibodies to tumor associated antigens have not become yet routine reagents in immunopathology, they have provided new information which could not be obtained with conventional antisera or histochemical procedures. The following are representative examples. TUmor associated antigens have been identified which display a restricted distribution in normal tissues and therefore may represent useful markers for radio­ imaging and appropriate targets for immunotherapy. In spite of undetec­ table differences with conventional histopathological approaches hetero­ geneity has been found in the antigenic profile of tumor cells within a lesion, in autologous lesions removed from different anatomic sites from a given patient and in lesions removed from different patients. Phenotypes of tumor cells have been identified which correlate with the biology of tumor cells and with the clinical course of the disease. From a practical view point the use of monoclonal antibodies in immunopathology has enhanced interactions between pathologists and immunologists, as exemplified by the present book. Such interactions have contributed to the application of basic research to clinical problems. The chapter of this book discuss investigations performed with monoclonal antibodies to antigens expressed by various types of normal and malignant human cells.

• Antigen
• antibody
• carcinoma
• cell
• classification
• histopathology
• liver
• lymphoma
• melanoma
• mesothelioma
• oncogene
• ovarian cancer
• pathology
• proteins
• tumor

• Department of Pathology, University Medical Center, Nijmegen, The Netherlands

  Dirk J. Ruiter

• Department of Pathology, University Medical Center, Leiden, The Netherlands

  Gert Jan Fleuren, Sven O. Warnaar

Policies and ethics