Handbook of Capillary Electrophoresis Applications

1. Front Matter

   Pages i-xxiv

   PDF

2. Introduction

   1. Introduction

      • K. Kitagishi

      Pages 1-14

3. Equipment Systems

   1. Front Matter

      Pages 15-15

      PDF

   2. Sample injection

      • K. Kitagishi

      Pages 17-24

   3. Quantitative analysis, precision and reproducibility

      • K. Kitagishi

      Pages 25-40

   4. Precision and quantitation in capillary electrophoresis

      • G. A. Ross

      Pages 41-55

   5. Column technology

      • K. Kitagishi

      Pages 56-70

   6. Electrolyte systems

      • K. Kitagishi

      Pages 71-83

   7. Detection

      • K. Kitagishi

      Pages 84-103

   8. Recent advances in fluorescence detection technology

      • M. Saito, Y. Kurosu

      Pages 104-117

   9. Other systems

      • K. Kitagishi

      Pages 118-121

   10. Advanced systems

       • K. Kitagishi

       Pages 122-127

   11. Automated fraction collection in capillary electrophoresis

       • R. Grimm

       Pages 128-136

   12. Comparison with other analytical methods

       • K. Kitagishi

       Pages 137-146

4. Biochemistry Applications

   1. Front Matter

      Pages 147-147

      PDF

   2. Amino acids

      • L. J. Brunner

      Pages 149-157

   3. Sodium dodecyl sulfate (SDS)-non-gel molecular sieving capillary electrophoresis for proteins

      • Y. Kurosu, M. Saito

      Pages 158-172

   4. Protein analysis by capillary electrophoresis

      • F.-T. A. Chen, R. A. Evangelista

      Pages 173-197

   5. Analysis of erythropoietin by capillary electrophoresis

      • F.-T. A. Chen

      Pages 198-206

   6. Assay of enzymes by capillary electrophoresis

      • F.-T. A. Chen, R. A. Evangelista

      Pages 207-218

Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance.
The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

• biochemistry
• cancer
• drug
• mutation
• research

• National Institute of Health Sciences, Ministry of Health & Welfare, Tokyo, Japan

  Hideharu Shintani

• Slovak Technical University, Bratislava, Slovakia

  J. Polonský

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