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Photochemical Probes in Biochemistry

  • Book
  • © 1989

Overview

Editors:
  1. Peter E. Nielsen

    1. Department of Biochemistry B, University of Copenhagen The Panum Institute, Copenhagen, Denmark

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Part of the book series: Nato Science Series C: (ASIC, volume 272)

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Table of contents (21 chapters)

  1. Front Matter

    Pages i-ix
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  2. Photolabeling Reagent Design

    • Ole Buchardt, Ulla Henriksen, Troels Koch, Peter E. Nielsen
    Pages 1-9

  3. Nucleophilic Aromatic Photo Substitutions on Nitrophenyl Ethers. A New Photoaffinity Labelling Technique?

    • Jorge Marquet, Marcial Moreno-Mañas
    Pages 11-29

  4. Photochemistry of Aryl and Aroyl Azides: Application to Photolabeling of Biological Systems

    • Gary B. Schuster
    Pages 31-41

  5. Membrane Protein Labelling with Photoreactive Phospholipid Analogues

    • Cesare Montecucco, Giampietro Schiavo, Emanuele Papini, Maurizio Tomasi, Roberto Bisson
    Pages 43-58

  6. 125 J-ASA-PE, A Photoactivable, Radioactive Phospholipid-Analogue, Designed for Photolabeling Proteins in Contact with Phospholipid Head Groups

    • Zhan Gao, Edmund Baeuerlein
    Pages 59-65

  7. Photoaffinity Labelling of Receptors for Melanocyte-Stimulating Hormone

    • A. N. Eberle, F. Solca, W. Siegrist, T. Scimonelli, J. Girard, P. N. E. de Graan
    Pages 67-84

  8. Topographical Analysis of the Torpedo Marmorata Acetylcholine Receptor by Energy Transfer Photoaffinity Labeling Using Aryldiazonium Derivatives

    • F. Kotzyba-Hibert, A. Jaganathen, J. Langenbuch-Cachat, M. Goeldner, C. Hirth, M. Dennis et al.
    Pages 85-105

  9. Photosuicide Labelling

    • L. Ehret-Sabatier, B. Kieffer, M. P. Goeldner, C. G. Hirth
    Pages 107-122

  10. Antibiotic Photoaffinity Labeling Probes of Escherichia Coli Ribosomal Structure and Function

    • Barry S. Cooperman, Melissa A. Buck, Carmen L. Fernandez, Carl J. Weitzmann, Barbara F. D. Ghrist
    Pages 123-139

  11. Dissection of the Ribosomal Peptidyl Transferase Center by Photoaffinity Labeling: P- And A-Sites Are Located At Different Positions in Domain V of 23 S RNA

    • E. Kuechler, G. Steiner, A. Barta
    Pages 141-156

  12. Studying the Cytoskeleton by Label Transfer Crosslinking: Uses and Limitations

    • Martin Alexander Schwartz
    Pages 157-168

  13. RecA-Directed Hybridization of Psoralen-Monoadducted DNA Oligonucleotides to Duplex Targets

    • Suzanne Cheng, Howard B. Gamper, John E. Hearst
    Pages 169-177

  14. The use of Psoralen-Photocrosslinking for the Analysis of the Chromatin Structure during Transcription

    • J. M. Sogo, A. Conconi, R. M. Widmer
    Pages 179-194

  15. Metal Complexes as Photochemical Probes of DNA Structure

    • Jacqueline K. Barton
    Pages 195-197

  16. Laser-Induced Strand Break Formation of Polyuridylic Acid in the Presence and Absence of Tris(2,2’-Bipyridyl)Ruthenium Chloride and K2S2O8

    • D. Schulte-Frohlinde, A. B. Tossi, H. Görner
    Pages 199-208

  17. Photonucleases

    • Ole Buchardt, Gunnar Karup, Michael Egholm, Troels Koch, Ulla Henriksen, Morten Meldal et al.
    Pages 209-218

  18. Sequence-Targeted Photochemical Reactions in Single-Stranded and Double-Stranded Nucleic Acids by Oligonucleotide-Photosensitizer Conjugates

    • C. Hélène, T. Le Doan, N. T. Thuong
    Pages 219-229

  19. Photofootprinting Analysis of Protein-DNA Interactions

    • Peter E. Nielsen, Claus Jeppesen
    Pages 231-240

  20. Studies of the Binding and Biological Actions of Ethidium

    • K. Lemone Yielding
    Pages 241-259
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Keywords

About this book

The concept of using photochemical probes in the study of biological systems was developed by Westheimer who published the first photoaffinity labeling experiments more than twenty years ago (J.Bio1. Chem. 237, (1962) 3006). Since then the concept has been used successfully in various areas of biochemistry and recently several new interesting and exciting aspects of the concept have been developed. It is the general opinion by scientists in the "field" that the full potential of photochemical probes in biochemical studies has far from been exploited yet. This is mostly due to the interdisciplinary character of the concept involving photochemistry, synthetic chemistry as well as biochemistry/molecular biology. The perspective of the NATO advanced workshop on "Photochemical Probes in Biochemistry", held in Holte (Copenhagen) Denmark 14-19, August, 1988, was several fold. The workshop was to give an account of the "state of the art" of using photochemical probes in biochemistry as well as to bring together specialists in photochemistry, syn­ thetic chemistry and molecular biology in order to analyze advantages as well as the inherent problems and pitfalls of the concept and provide suggestions and guidelines for fu­ ture research. Furthermore, it is the hope of the editor that the present publication which gives an account of the lectures presented at the workshop, will provide an introduction to scientists who are not familiar with photochemical probes, but to whom these could help answer central and pertinent questions.

Editors and Affiliations

  • Department of Biochemistry B, University of Copenhagen The Panum Institute, Copenhagen, Denmark

    Peter E. Nielsen

Bibliographic Information

  • Book Title: Photochemical Probes in Biochemistry

  • Editors: Peter E. Nielsen

  • Series Title: Nato Science Series C:

  • DOI: https://doi.org/10.1007/978-94-009-0925-0

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Kluwer Academic Publishers 1989

  • Hardcover ISBN: 978-0-7923-0171-4Due: 30 April 1989

  • Softcover ISBN: 978-94-010-6905-2Published: 22 September 2011

  • eBook ISBN: 978-94-009-0925-0Published: 06 December 2012

  • Series ISSN: 1389-2185

  • Edition Number: 1

  • Number of Pages: 320

  • Topics: Biochemistry, general, Organic Chemistry

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