Site-directed insertion of transgenes

1. Front Matter

   Pages i-xiii

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2. Site-Directed Integration of Transgenes

   1. Front Matter

      Pages 1-1

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3. Site-directed integration of transgenes

   1. Transgene Site-Specific Integration: Problems and Solutions

      • Solenne Bire, Florence Rouleux-Bonnin

      Pages 3-39

   2. Designing Non-viral Targeted Integrating Vectors for Genome Engineering in Vertebrates

      • Ludivine Sinzelle, Nicolas Pollet

      Pages 41-67

4. Integration Based on Homologous Recombination

   1. Front Matter

      Pages 69-69

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5. Integration based on homologous recombination

   1. Gene Targeting and Homologous Recombination in Saccharomyces cerevisiae

      • Judith Miné-Hattab, Rodney Rothstein

      Pages 71-89

   2. Homologous Recombination in Mammals

      • Aurélia Barascu, Anastazja Grabarz, Bernard S. Lopez

      Pages 91-120

   3. Engineered Zinc Finger Nucleases for Targeted Genome Editing

      • Cherie L. Ramirez, J. Keith Joung

      Pages 121-145

   4. Engineered Meganucleases for Genome Engineering Purposes

      • Jean Charles Epinat, George H. Silva, Frédéric Pâques, Julianne Smith, Philippe Duchateau

      Pages 147-185

6. Integration Based on Site-Specific Recombination

   1. Front Matter

      Pages 187-187

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7. Integration based on Site-specific recombination

   1. Cre/loxP, Flp/FRT Systems and Pluripotent Stem Cell Lines

      • Candice G. T. Tahimic, Kenji Sakurai, Kazuhiro Aiba, Norio Nakatsuji

      Pages 189-209

   2. Site-Specific Recombination Using PhiC31 Integrase

      • Jonathan M. Geisinger, Michele P. Calos

      Pages 211-239

   3. Modified Transposases for Site-Directed Insertion of Transgenes

      • Sean Colloms, Sylvaine Renault

      Pages 241-265

   4. Targeted Plasmid Integration into the Human Genome by Engineered Recombinases

      • Charles A. Gersbach, Carlos F. Barbas III

      Pages 267-284

8. Applications

   1. Front Matter

      Pages 285-285

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   2. Gene Site-Specific Insertion in Plants

      • Olivier Da Ines, Charles I. White

      Pages 287-315

   3. Site-Directed Insertion of Transgenes in Drosophila

      • Dana Carroll

      Pages 317-326

   4. Nuclease Mediated Targeted Genome Modification in Mammalian Cells

      • Jenny C. Barker, Richard A. Voit, Matthew H. Porteus

      Pages 327-352

   5. Bio-applications Derived from Site-Directed Genome Modification Technologies

      • Christophe Delenda, Sébastien Paris, Sylvain Arnould, Edward Balbirnie, Jean-Pierre Cabaniols

      Pages 353-384

9. Back Matter

   Pages 385-392

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The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.

• genome engineering
• homologous recombination
• site-specific recombination
• targeted genome modification
• transgenesis
• genetic engineering
• gene therapy
• gene expression

• , UFR des Sciences Pharmaceutiques, Université François Rabelais, Tours, France

  Sylvaine Renault

• Cellectis SA, Paris, France

  Philippe Duchateau

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