Overview
- Editors:
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Sylvaine Renault
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, UFR des Sciences Pharmaceutiques, Université François Rabelais, Tours, France
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Philippe Duchateau
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Cellectis SA, Paris, France
- No other book published in this field of research
- Presentation of all the innovative methodologies in genome engineering
- From basic science to practical applications
- Concerns a broad range of organisms (from bacteria to mammals)
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Table of contents (14 chapters)
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Front Matter
Pages i-xiii
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Site-Directed Integration of Transgenes
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Site-directed integration of transgenes
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- Solenne Bire, Florence Rouleux-Bonnin
Pages 3-39
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- Ludivine Sinzelle, Nicolas Pollet
Pages 41-67
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Integration Based on Homologous Recombination
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Integration based on homologous recombination
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- Judith Miné-Hattab, Rodney Rothstein
Pages 71-89
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- Aurélia Barascu, Anastazja Grabarz, Bernard S. Lopez
Pages 91-120
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- Cherie L. Ramirez, J. Keith Joung
Pages 121-145
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- Jean Charles Epinat, George H. Silva, Frédéric Pâques, Julianne Smith, Philippe Duchateau
Pages 147-185
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Integration Based on Site-Specific Recombination
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Front Matter
Pages 187-187
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Integration based on Site-specific recombination
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- Candice G. T. Tahimic, Kenji Sakurai, Kazuhiro Aiba, Norio Nakatsuji
Pages 189-209
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- Jonathan M. Geisinger, Michele P. Calos
Pages 211-239
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- Sean Colloms, Sylvaine Renault
Pages 241-265
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- Charles A. Gersbach, Carlos F. Barbas III
Pages 267-284
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Applications
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Front Matter
Pages 285-285
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- Olivier Da Ines, Charles I. White
Pages 287-315
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- Jenny C. Barker, Richard A. Voit, Matthew H. Porteus
Pages 327-352
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- Christophe Delenda, Sébastien Paris, Sylvain Arnould, Edward Balbirnie, Jean-Pierre Cabaniols
Pages 353-384
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Back Matter
Pages 385-392
About this book
The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.
Editors and Affiliations
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, UFR des Sciences Pharmaceutiques, Université François Rabelais, Tours, France
Sylvaine Renault
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Cellectis SA, Paris, France
Philippe Duchateau