Macromolecular Crystallography

1. Front Matter

   Pages i-xiv

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2. Spatial and Temporal Organisation of Multiprotein Systems of Cell Regulation and Signalling: What Can We Learn from NHEJ System of Double-Strand Break Repair?

   • Qian Wu, Lynn Sibanda, Takashi Ochi, Victor M. Bolanos-Garcia, Tom L. Blundell, Dimitri Y. Chirgadze

   Pages 1-31

3. Co-translational Protein Processing, Folding, Targeting, and Membrane Insertion of Newly Synthesized Proteins

   • Daniel Boehringer, Nenad Ban

   Pages 33-41

4. The Role of Multiple Sequence Repeat Motifs in the Assembly of Multi-protein Complexes

   • David Barford

   Pages 43-49

5. Cryoelectron Tomography or Doing Structural Biology In Situ

   • Wolfgang Baumeister

   Pages 51-54

6. RuvBL1 and RuvBL2 and Their Complex Proteins Implicated in Many Cellular Pathways

   • Sabine Gorynia, Tiago M. Bandeiras, Pedro M. Matias, Filipa G. Pinho, Colin E. McVey, Peter Donner et al.

   Pages 55-63

7. The Structural Biology of Muscle: Spatial and Temporal Aspects

   • Kenneth C. Holmes

   Pages 65-78

8. Molecular Basis of Allosteric Transitions: GroEL

   • Amnon Horovitz

   Pages 79-86

9. Cell Signalling Through Covalent Modification and Allostery

   • Louise N. Johnson

   Pages 87-92

10. Combining Cryo-EM and X-ray Crystallography to Study Membrane Protein Structure and Function

    • Werner Kühlbrandt

    Pages 93-101

11. Molecular Mechanisms of DNA Polymerase Clamp Loaders

    • Brian Kelch, Debora Makino, Kyle Simonetta, Mike O’Donnell, John Kuriyan

    Pages 103-114

12. Electron Microscopy of Macromolecular Machines

    • Helen R. Saibil

    Pages 115-123

13. Assembly and Function of the Signal Recognition Particle from Archaea

    • Elisabeth Sauer-Eriksson, Shenghua Huang, Tobias Hainzl

    Pages 125-133

14. Structural Studies of the Functional Complexes of the 50S and 70S Ribosome, a Major Antibiotic Target

    • Thomas A. Steitz, Gregor Blaha, C. Axel Innis, Robin Evans Stanley, David Bulkley

    Pages 135-148

15. Proteopedia: Exciting Advances in the 3D Encyclopedia of Biomolecular Structure

    • Jaime Prilusky, Eran Hodis, Joel L. Sussman

    Pages 149-161

16. Structure Analysis of Biological Macromolecules by Small-Angle X-ray Scattering

    • Dmitri I. Svergun

    Pages 163-171

17. Structural Dynamics of the Vault Ribonucleoprotein Particle

    • Arnau Casañas, Jordi Querol, Ignasi Fita, Núria Verdaguer

    Pages 173-182

18. Structural Dynamics of Picornaviral RdRP Complexes. Implications for the Design of Antivirals

    • Núria Verdaguer, Cristina Ferrer-Orta, Esteban Domingo

    Pages 183-193

19. Ribosomes: Ribozymes that Survived Evolution Pressures but Is Paralyzed by Tiny Antibiotics

    • Ada Yonath

    Pages 195-208

This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics.
A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.

• Assembly of Multiprotein Complexes
• Cell regulation and signalling
• Co-translational processing
• Combining Cryo-EM and X-Ray Crystallography
• Three-dimensional reconstruction
• folding
• structural biology
• targeting Transcriptional regulation
• Protein-Ligand Interactions
• Protein Structure

• , Instit.de Technologia Química e Biológia, Universidade Nova de Lisboa, Oeiras, Portugal

  Maria Armenia Carrondo

• Dipto. Scienze Chimiche, Università di Padova, Padova, Italy

  Paola Spadon

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