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Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

  • Conference proceedings
  • © 2012

Overview

Editors:
  1. Nigel Jenkins

    1. , University College Dublin, National Institute of Bioprocessing Rese, Dublin, Ireland

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  2. Niall Barron

    1. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  3. Paula Alves

    1. Tecnologica (IBET), ITQB, Instituto de Biologia Experimental e, Oeiras, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  • A comprehensive review of the latest research in animal cell culture and technology
  • This book includes unique papers on animal cell technology from an industrial point of view
  • Provides the latest practical information on the applications of animal cell technology in the production of biomedicines

Part of the book series: ESACT Proceedings (ESACT, volume 5)

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Table of contents (119 papers)

  1. Front Matter

    Pages i-xliv
    Download chapter PDF

  2. Synthetic and Molecular Biology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes

      • Niall Barron, Niraj Kumar, Noelia Sanchez, Patrick Gammell, Martin Clynes
      Pages 3-11

    3. Are Clones Really Unstable?

      • Kriti Shukla, Saravanan Desan, Ankur Bhatnagar, Anuj Goel
      Pages 13-18

    4. An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media

      • Joe Codamo, Trent P. Munro, Benjamin S. Hughes, Michael Song, Peter P. Gray
      Pages 19-23

    5. Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium

      • K.R. de la Luz-Hernández, Y. Rabasa-Legón, A. Lage-Castellanos, A. Castillo-Vitlloch, L. Castellanos-Serra, J. Díaz-Brito et al.
      Pages 25-29

    6. Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells

      • Fanny Delegrange, Mattia Matasci, Lucia Baldi, Florian M. Wurm
      Pages 31-35

    7. Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells

      • Stefanie Dietmair, Nicholas E. Timmins, Panagiotis Chrysanthopoulos, Peter P. Gray, Jens O. Krömer, Lars K. Nielsen
      Pages 37-41

    8. Cell Lines in Four Weeks with the CEMAX® System

      • Benedikt Greulich, Karlheinz Landauer, Andreas Herrmann
      Pages 43-46

    9. Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination

      • Jeff Jia Cheng Hou, Michael Song, Trent P. Munro, Peter P. Gray
      Pages 47-51

    10. A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand

      • Kirin M. Jamison, Dacia R. Brooks, Szu-Han Wang, Martin Gawlitzek
      Pages 53-56

    11. An Improved Clone Selection Method

      • Uros Jamnikar, Marjanca Blas, Dominik Gaser, Jerica Rozman-Pungercar, Andrej Francky
      Pages 57-60

    12. Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines

      • Ernest Milián, Sandra Juanola, Eva Prats, Jordi J. Cairó, Francesc Gòdia, Joaquim Vives
      Pages 61-65

    13. Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems

      • Anne Marie Molloy, Patrick Dowling, Mairead Looby, Caitriona Crawford, Bruce Tangarone, Mary Heenan et al.
      Pages 67-70

    14. Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance

      • Sophie Nallet, Zuzana Kadlecova, Lucia Baldi, Harm-Anton Klok, Florian M. Wurm
      Pages 71-75

    15. Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX

      • Say Kong Ng, Wenyu Lin, Rohit Sachdeva, Daniel I.C. Wang, Miranda G.S. Yap
      Pages 77-83

    16. Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines

      • Y. Prieto, L. Rojas, L. Hinojosa, K.R. de la Luz-Hernández, D. Aguiar, S. Victores et al.
      Pages 85-90

    17. A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells

      • Yashas Rajendra, Gaurav Backliwal, Markus Hildinger, Sarah Wulhfard, Maria de Jesus, Florian M. Wurm
      Pages 91-94

    18. Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture

      • Niraj Kumar, Noelia Sanchez, Patrick Gammell, Patrick Dowling, M. Henry, P. Meleady et al.
      Pages 95-107

    19. Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy

      • Kristina Nehlsen, Jeannette Zauers, Sabrina Herrmann, Hansjörg Hauser, Dagmar Wirth
      Pages 109-113
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About this book

The 21st ESACT conference was held in the beautiful surroundings of the CityWest Hotel resort in Dublin, Ireland. For the first time in ESACT history the number of participants exceeded 900: a sign of the ever increasing importance of this area. The conference commenced on Sunday June 5th with two sets of parallel workshops on the subjects listed below. An additional workshop was held on Monday lunchtime of the conferenceProcess Analytical Technology (PAT), Quality by Design (QbD) and other recent regulatory developments.

2.      Innovative media products for the 21st century biopharmaceutical industry.

3.      The impact of high titre media feed-streams on monoclonal antibody purification.

4.      Advances in genomics and proteomics.

5.      Stem Cell Technology: new developments and clinical applications.

Editors and Affiliations

  • , University College Dublin, National Institute of Bioprocessing Rese, Dublin, Ireland

    Nigel Jenkins

  • , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

  • Tecnologica (IBET), ITQB, Instituto de Biologia Experimental e, Oeiras, Portugal

    Paula Alves

Bibliographic Information

  • Book Title: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

  • Editors: Nigel Jenkins, Niall Barron, Paula Alves

  • Series Title: ESACT Proceedings

  • DOI: https://doi.org/10.1007/978-94-007-0884-6

  • Publisher: Springer Dordrecht

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Science+Business Media B.V. 2012

  • Hardcover ISBN: 978-94-007-0883-9Published: 15 September 2011

  • Softcover ISBN: 978-94-007-3806-5Published: 27 November 2013

  • eBook ISBN: 978-94-007-0884-6Published: 15 September 2011

  • Edition Number: 1

  • Number of Pages: XLIV, 756

  • Topics: Biomedicine general, Cell Biology, Life Sciences, general, Biotechnology

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