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Invertebrate Tissue Culture Methods

  • Book
  • © 2002

Overview

Authors:
  1. Jun Mitsuhashi

    1. Tokyo University of Agriculture, Setagaya, Tokyo, Japan

    View author publications

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  • With step-by-step protocols, it introduces methods to initiate tissue culture from invertebrates including, Polifera, Coelenterata, Plathelminthes, Nematoda, Annedlia, Mollusca, Echinodermata, and Prochordata
  • The Appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (42 chapters)

  1. Front Matter

    Pages I-XVI
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  2. General Methods

    1. Front Matter

      Pages 1-1
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    2. Facilities and Equipment

      • Jun Mitsuhashi
      Pages 3-14

    3. Basic Information and Overview

      • Jun Mitsuhashi
      Pages 15-24

    4. Preparation of Media

      • Jun Mitsuhashi
      Pages 25-32

    5. General Cell Culture Methods

      • Jun Mitsuhashi
      Pages 33-44

  3. Methods for Setting Up Primary Cultures Specific to Animal Groups

    1. Front Matter

      Pages 45-45
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    2. Insecta (Lepidoptera)

      • Jun Mitsuhashi
      Pages 47-71

    3. Insecta (Diptera)

      • Jun Mitsuhashi
      Pages 73-86

    4. Insecta (Coleoptera)

      • Jun Mitsuhashi
      Pages 87-90

    5. Insecta (Hemiptera)

      • Jun Mitsuhashi
      Pages 91-99

    6. Insecta (Hymenoptera)

      • Jun Mitsuhashi
      Pages 101-105

    7. Insecta (Blattaria)

      • Jun Mitsuhashi
      Pages 107-115

    8. Insecta (Orthoptera)

      • Jun Mitsuhashi
      Pages 117-120

    9. Arthropods Other Than Insects

      • Jun Mitsuhashi
      Pages 121-141

    10. Prochordata

      • Jun Mitsuhashi
      Pages 143-151

    11. Echinodermata

      • Jun Mitsuhashi
      Pages 153-160

    12. Mollusca

      • Jun Mitsuhashi
      Pages 161-195

    13. Annelida

      • Jun Mitsuhashi
      Pages 197-201

    14. Nematoda

      • Jun Mitsuhashi
      Pages 203-207
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Keywords

About this book

I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow­ ing cells from pupal ovaries of the emperor gum moth, Antheraea euca­ lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul­ tured. The vertebrate tissue culture methods gave me some ideas for pre­ paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec­ ond, I had to examine various culture media, especially growth-promot­ ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Authors and Affiliations

  • Tokyo University of Agriculture, Setagaya, Tokyo, Japan

    Jun Mitsuhashi

Bibliographic Information

  • Book Title: Invertebrate Tissue Culture Methods

  • Authors: Jun Mitsuhashi

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-4-431-67875-5

  • Publisher: Springer Tokyo

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Tokyo 2002

  • Softcover ISBN: 978-4-431-70313-6Published: 01 February 2002

  • eBook ISBN: 978-4-431-67875-5Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XVI, 446

  • Number of Illustrations: 121 b/w illustrations

  • Topics: Cell Biology, Zoology

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