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Analytical Ultracentrifugation

Instrumentation, Software, and Applications

  • Book
  • © 2016

Overview

Editors:
  1. Susumu Uchiyama

    1. Graduate School of Engineering, Osaka University, Suita, Japan

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  2. Fumio Arisaka

    1. Life Science Research Center, Nihon University, Fujisawa, Japan

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    You can also search for this editor in PubMed   Google Scholar

  3. Walter F. Stafford

    1. Department of Neurology, Harvard Medical School, Boston, USA

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    You can also search for this editor in PubMed   Google Scholar

  4. Tom Laue

    1. BITC, University of New Hampshire, Durham, USA

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  • Covers recent advances in AUC studies comprehensively
  • Includes knowledge on hardware, software, and applications
  • Provides essential knowledge for readers who are engaged in the practical use of AUC in industries

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Table of contents (26 chapters)

  1. Front Matter

    Pages i-ix
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Important and Essential Theoretical Aspects of AUC

      • Susumu Uchiyama, Fumio Arisaka
      Pages 3-14

    3. Experimental Design and Practical Aspects

      • Fumio Arisaka, Susumu Uchiyama
      Pages 15-21

  3. AUC Instrumentation and Analysis

    1. Front Matter

      Pages 23-23
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    2. The CFA Analytical Ultracentrifuge Architecture

      • Thomas M. Laue, J. Brett Austin
      Pages 25-37

    3. Fluorescence Detection System

      • Tao G. Nelson, Glen D. Ramsay, Matthew A. Perugini
      Pages 39-61

    4. The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension

      • Engin Karabudak, Helmut Cölfen
      Pages 63-80

    5. SEDANAL: Model-Dependent and Model-Independent Analysis of Sedimentation Data

      • Peter J. Sherwood, Walter F. Stafford
      Pages 81-102

    6. SEDANAL: Global Analysis of General Hetero- and Self-Associating Systems by Sedimentation Equilibrium

      • Walter F. Stafford, Peter J. Sherwood
      Pages 103-117

    7. Analytical Ultracentrifugation Data Analysis with UltraScan-III

      • Borries Demeler, Gary E. Gorbet
      Pages 119-143

  5. Applications of AUC: Material Science

    1. Front Matter

      Pages 249-249
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    2. Hydrodynamic Analysis of Synthetic Permanently Charged Polyelectrolytes

      • Christine Wandrey, Hamideh Ahmadloo
      Pages 251-268

    3. Different Levels of Self-Sufficiency of the Velocity Sedimentation Method in the Study of Linear Macromolecules

      • Georges M. Pavlov
      Pages 269-307

  6. Applications of AUC: Biological Science

    1. Front Matter

      Pages 309-309
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Keywords

About this book

This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC.
This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included.
The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.

Editors and Affiliations

  • Graduate School of Engineering, Osaka University, Suita, Japan

    Susumu Uchiyama

  • Life Science Research Center, Nihon University, Fujisawa, Japan

    Fumio Arisaka

  • Department of Neurology, Harvard Medical School, Boston, USA

    Walter F. Stafford

  • BITC, University of New Hampshire, Durham, USA

    Tom Laue

About the editors

Susumu Uchiyama, Ph. D., Associate Professor, Department of Biotechnology, Graduate School of Engineering, Osaka University, 2‐1 Yamadaoka, Suita, Osaka 565‐0871, Japan

Fumio Arisaka, Ph. D., Professor, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori‐ku, Yokohama 226‐8501, Japan

Walter F. Stafford, III, Ph.D., Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115, USA

Tom Laue, Ph.D., Professor, Biomolecular Interaction Technologies Center (BITC), Center to Advance Molecular Interaction Science (CAMIS), University of New Hampshire, Rudman Hall, Room 379, 46 College
Road, Durham, NH 03824, USA

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