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In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

  • Book
  • © 2016

Overview

Editors:
  1. Shinichi Ohno

    1. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

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  2. Nobuhiko Ohno

    1. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

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  3. Nobuo Terada

    1. Division of Health Science, Shinshu University Graduate School of Medicine, Nagano, Japan

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  • Presents applications of a unique cryotechnique, in vivo cryotechnique

  • Is written for both biomedical researchers and clinical doctors interested in morphology

  • Covers the latest trends in live imaging, immunohistochemistry, gene manipulation and new microscopy analysis

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Table of contents (54 chapters)

  1. Front Matter

    Pages i-xi
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  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Introduction

      • Shinichi Ohno
      Pages 3-5

    3. Biomedical Significance and Development of “IVCT”

      • Shinichi Ohno
      Pages 7-10

    4. How to Perform “IVCT”

      • Shinichi Ohno
      Pages 11-14

    5. Technical Merits with “IVCT”

      • Shinichi Ohno
      Pages 15-16

  3. Application of ‘IVCT’ to Various Mouse Organs

    1. Front Matter

      Pages 17-17
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    2. Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions

      • Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 19-23

    3. Three-Dimensional Reconstruction of Liver Tissues with Confocal Laser Scanning Microscopy

      • Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 25-28

    4. Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole

      • Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 29-32

    5. Immunohistochemical Detection of Soluble Immunoglobulins in Small Intestines

      • Satoshi Shimo, Sei Saitoh, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 33-38

    6. Detection of MAPK Signal Transduction Proteins in an Ischemia/Reperfusion Model of Small Intestines

      • Jiaorong Chen, Nobuo Terada, Yurika Saitoh, Zheng Huang, Nobuhiko Ohno, Shinichi Ohno
      Pages 39-44

    7. Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes

      • Yuqin Bai, Bao Wu, Nobuo Terada, Nobuhiko Ohno, Yurika Saitoh, Shinichi Ohno
      Pages 45-49

    8. Distribution of Immunoglobulin-Producing Cells in Immunized Mouse Spleens

      • Sei Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 51-56

    9. Alteration of Erythrocyte Shapes in Various Organs of Living Mice or Under Blood Flow Conditions

      • Shinichi Ohno, Nobuo Terada, Yasuhisa Fujii
      Pages 57-61

    10. Dynamic Ultrastructure of Smooth Muscle Cells in Dystrophin-Deficient mdx or Normal scn Mice

      • Shinichi Ohno
      Pages 63-66

    11. Dynamic Bioimaging of Serum Proteins in Beating Mouse Hearts

      • Liye Shi, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 67-76

    12. Histochemical Analyses and Quantum Dot Imaging of Microvascular Blood Flow with Pulmonary Edema

      • Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 77-81

    13. Dynamic Ultrastructure of Pulmonary Alveoli of Living Mice Under Respiratory Conditions

      • Shinichi Ohno, Nobuo Terada, Yasuhisa Fujii
      Pages 83-86

    14. Immunohistochemical Analysis of Various Serum Proteins in Living Mouse Thymus

      • Yuqin Bai, Bao Wu, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Sei Saitoh et al.
      Pages 87-92
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Keywords

About this book

This book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.

Editors and Affiliations

  • Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

    Shinichi Ohno, Nobuhiko Ohno

  • Division of Health Science, Shinshu University Graduate School of Medicine, Nagano, Japan

    Nobuo Terada

About the editors

Shinichi Ohno, M.D., Ph.D.

Professor

Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

Nobuhiko Ohno, M.D., Ph.D.

Associate Professor

Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

Nobuo Terada, M.D., Ph.D.

Professor

Division of Health Science, Shinshu University Graduate School of Medicine

Bibliographic Information

  • Book Title: In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

  • Editors: Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada

  • DOI: https://doi.org/10.1007/978-4-431-55723-4

  • Publisher: Springer Tokyo

  • eBook Packages: Medicine, Medicine (R0)

  • Copyright Information: Springer Japan 2016

  • Hardcover ISBN: 978-4-431-55722-7Published: 07 December 2015

  • Softcover ISBN: 978-4-431-56684-7Published: 29 March 2019

  • eBook ISBN: 978-4-431-55723-4Published: 26 November 2015

  • Edition Number: 1

  • Number of Pages: XI, 300

  • Number of Illustrations: 15 b/w illustrations, 99 illustrations in colour

  • Topics: Anatomy, Medical Biochemistry, Biological Microscopy

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