Overview
- Detailed descriptions of standard, essential, and widely used patch clamp (PC) techniques.
- Practical descriptions of PC techniques for beginners and recently developed variations for experts.
- The most up-to-date applications of PC, useful in academia and industry.
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (28 protocols)
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Front Matter
Keywords
About this book
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Reviews
From the reviews:
“This book is the most current single ‘how to’ manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. … The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons.” (Joseph J. Grenier, Amazon.com, May, 2014)
Editors and Affiliations
Bibliographic Information
Book Title: Patch Clamp Techniques
Book Subtitle: From Beginning to Advanced Protocols
Editors: Yasunobu Okada
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-4-431-53993-3
Publisher: Springer Tokyo
eBook Packages: Springer Protocols
Copyright Information: Springer 2012
Hardcover ISBN: 978-4-431-53992-6Published: 27 April 2012
Softcover ISBN: 978-4-431-56105-7Published: 23 August 2016
eBook ISBN: 978-4-431-53993-3Published: 30 March 2012
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: XI, 439
Topics: Neurosciences, Human Physiology, Cell Biology, Biological and Medical Physics, Biophysics, Animal Physiology, Pharmacology/Toxicology