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Table of contents (27 chapters)
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Electroporation
Keywords
About this book
In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.
Editors and Affiliations
Bibliographic Information
Book Title: Electroporation and Sonoporation in Developmental Biology
Editors: Harukazu Nakamura
DOI: https://doi.org/10.1007/978-4-431-09427-2
Publisher: Springer Tokyo
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer-Verlag Tokyo 2009
Softcover ISBN: 978-4-431-09426-5Published: 17 February 2009
eBook ISBN: 978-4-431-09427-2Published: 31 January 2009
Edition Number: 1
Number of Pages: XXIX, 331
Topics: Developmental Biology, Cell Biology, Neurobiology, Animal Genetics and Genomics, Biotechnology, Genetic Engineering