Overview
- Editors:
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Thomas Hohn
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Friedrich Miescher-Institut, Basel, Switzerland
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Joszef Schell
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Max-Planch-Institut für Züchtungsforschung, Köln, Germany
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Table of contents (15 chapters)
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- J. W. Davies, R. Townsend, J. Stanley
Pages 31-52
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- Nigel Grimsley, David Bisaro
Pages 87-107
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- Zdena Koukolíková-Nicola, Lisa Albright, Barbara Hohn
Pages 109-148
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- Frank F. White, Vilas P. Sinkar
Pages 149-177
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- Stephen G. Rogers, Harry Klee
Pages 179-203
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- I. Potrykus, J. Paszkowski, R. D. Shillito, M. W. Saul
Pages 229-247
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- Brian L. A. Miki, Terry J. Reich, V. N. Iyer
Pages 249-265
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- H.-G. Schweiger, G. Neuhaus
Pages 285-302
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- Michael Fromm, Virginia Walbot
Pages 303-310
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- M. J. Cornelissen, M. De Block, M. Van Montagu, J. Leemans, P. H. Schreier, J. Schell
Pages 311-320
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- Les J. Szabo, Anthony R. Cashmore
Pages 321-339
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Back Matter
Pages 341-350
About this book
There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans forming genes can be studied in plant cells during "transient expression".
Editors and Affiliations
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Friedrich Miescher-Institut, Basel, Switzerland
Thomas Hohn
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Max-Planch-Institut für Züchtungsforschung, Köln, Germany
Joszef Schell
