Overview
- The FFF family of techniques applied to biopolymer analysis is covered for the first time
- Summarizes FFF technologies and approaches to date
- Experts in FFF contribute their experiences from diverse fields
- Regulatory approval considerations in FFF methods development are addressed
- Includes supplementary material: sn.pub/extras
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Table of contents (17 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.
Reviews
From the reviews:
“I recommend this book to every FFF user and analytical chemist, and to any researcher, post-graduate students, and academics working with biopolymers. … This book … is essential reading for every current and potential FFF user. It discusses, in depth, current and potential applications, the superiority of FFF over other methods … . This book is certainly a precious help for all scientists in the various fields of (biomedical) research, cell biology, food science, membrane technology, and environmental science, to name a few.” (Patrice Castignolles, Analytical and Bioanalytical Chemistry, Vol. 404, 2012)
Editors and Affiliations
Bibliographic Information
Book Title: Field-Flow Fractionation in Biopolymer Analysis
Editors: S. Kim R. Williams, Karin D. Caldwell
DOI: https://doi.org/10.1007/978-3-7091-0154-4
Publisher: Springer Vienna
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer-Verlag/Wien 2012
Hardcover ISBN: 978-3-7091-0153-7Published: 07 December 2011
Softcover ISBN: 978-3-7091-1719-4Published: 01 March 2014
eBook ISBN: 978-3-7091-0154-4Published: 03 December 2011
Edition Number: 1
Number of Pages: X, 306
Topics: Analytical Chemistry, Pharmaceutical Sciences/Technology, Protein Science, Medicinal Chemistry