Editors:
- Offers readily reproducible, step-by-step laboratory methods
- Provides helpful tips and tricks to complement the protocols
- Includes troubleshooting advice
- Features practical laboratory guidelines to promote successful results
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (14 protocols)
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Front Matter
About this book
This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitationof useful activities of such microbes.
Editors and Affiliations
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School of Biological Sciences, University of Essex Dept. Biological Sciences, Colchester, United Kingdom
Terry J. McGenity
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Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany
Kenneth N. Timmis
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Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain
Balbina Nogales
Bibliographic Information
Book Title: Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle: Bioproducts, Biofuels, Biocatalysts and Facilitating Tools
Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-662-53115-0
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag Berlin Heidelberg 2017
Hardcover ISBN: 978-3-662-53113-6Published: 16 December 2016
Softcover ISBN: 978-3-662-57114-9Published: 13 July 2018
eBook ISBN: 978-3-662-53115-0Published: 07 December 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 245
Number of Illustrations: 20 b/w illustrations, 30 illustrations in colour
Topics: Microbiology, Biochemical Engineering